Tespa1 (NM_183264) Mouse Untagged Clone

CAT#: MC216153

Tespa1 (untagged) - Mouse RIKEN cDNA 5830405N20 gene (5830405N20Rik), (10ug)


  "NM_183264" in other vectors (4)

Reconstitution Protocol

USD 503.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Tespa1
Synonyms 5830405N20Rik; A430001F24Rik; AI450549
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216153 representing NM_183264
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCATCGGTACTGAGCCCCACATCCTGGGAGAAACGAAGGGCCTGGTTACGACAGAGTCGTAACT
GGCAAACCCAGGTCCTGGAAGAGGAAGCAGCAGCAGCTCTTCAGGATGCACTGGATCCCGAGCCTTCTAG
CCTGGATGATGTTTTCCAGGAAGGGAATCCAATCAATAAGATTGAGGACTGGCTGCAGGGCTGTGGTTGT
AGAGACACTGAAGAGGGCCTTTCTGAAGAATCAGGGCAATCTAACTACAGTGGATATTCTAGCCATGGGA
CAAGCTTTGAAGATGACTTGAGTCTTGGAGCAGAGGCCACATTACTGTCTACCAATGGAAACCTCTTCTC
TAGGAATTTCCTCCAGACACCCAGGCTTTGCCAGTTACTTGATCTTGGCTCTAGTTTGGCTTCCAGCAGC
ATGACTGGTGGAACCAACAAAACCAGTTCAAGCATCTCAGAGATTCTGGACCAGGTACAAGAAGATGCAG
AAGATATTCTTTTCAGCCTGGGCTTCGGGCACGAGAACCACAAAGACACATCTCGTATCCCTGCAAGATT
TTTCAGCAACCCTTCTCAGGCCAAGGGCATTAACTTTCAGCTCTTCCTGAAGTCTCAGGTGCAAAGGATG
GAGATGGAGGATCCCTGCCTGATGCTGGCCAGCCGGTTTAAGCAGGTGCAGACGCTGGCTGTCACTGCTG
ATGCTTTCTTCTGTCTCTACTCCTATGTGTCTAAGACACCTGTCCAAAAGTTCACACCATCCAACATGTT
CTGGAATTTCGACCCAACGGATGTGCCATCCATCAGGATTCTGGCTCCAGAGCCTGAACCCTACTCACCC
AGAGAACGTCTCAGGAGAGCAATCTCAAAAATGTGCCTGTATACAGGTTCCCGAGATCGGCTATCATCAT
CTTACAACAACCCCAAAAAGAACAGTTTGGATCAAATTGTGTGGGAAGTGATGGACAGAGTGAAAGGAGA
GAAGATCCAGCAAGACCCTGAGCATAGGCAAGCCCTTGGGGAAGAGTCTGTTCCCCCAATACAAAATACA
AATCCATCTACTTCTTCCCTTCCATGTGTTTCCTACCCTAAAGAAGAAACACAGGGAGACATGTGCCATG
CTCATGCTCTAGCAAGACCAGGTCCACAGTACCACATAAACTCTACACAGGTAAGGAGACAGCTGTGGGT
TCTACAGGACATTAATGAGAAGCCACGTTCAGCTGAGAATGAGAGTCCATGGGAAAGAAAAAGCAAAGCA
AGAAAGAATCTGTTTCAAAGGGTTCCTGTGGACAAGAATATTAAGTCATTGAACTTGCCCACCATCCAGC
AGAAACAAAACCAGGGACAAGCCAGACATGAGCTGACCAATCTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_183264
Insert Size 1377 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_183264.4, NP_899087.2
RefSeq Size 1694 bp
RefSeq ORF 1377 bp
Locus ID 67596
UniProt ID Q3U132
Cytogenetics 10 D3
Gene Summary May play a role in the regulation of inositol 1,4,5-trisphosphate receptor-mediated Ca(2+) release and mitochondrial Ca(2+) uptake via the mitochondria-associated endoplasmic reticulum membrane (MAM) compartment (By similarity). Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development. Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.