Esrrb (NM_011934) Mouse Untagged Clone

CAT#: MC216139

Esrrb (untagged) - Mouse estrogen related receptor, beta (Esrrb), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Esrrb
• Synonyms: Err2; Errb; Estrrb; Nr3b2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216139 representing NM_011934
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGTCCGAACTCTGCATCCCGGACCCCCTTGGCTACCACAACCAGCTGCTGAACCGAATGTCGT
  CCGAAGACAGGCACCTGGGCTCTAGTTGCGGCTCCTTCATCAAGACGGAGCCATCCAGCCCGTCCTCGGG
  CATTGATGCCCTCAGCCACCACAGCCCCAGCGGCTCGTCGGACGCCAGTGGTGGCTTTGGCATTGCCCTG
  AGCACCCACGCCAACGGTCTGGACTCGCCGCCTATGTTCGCAGGTGCGGGGCTGGGAGGCAACCCGTGCC
  GCAAGAGCTACGAGGACTGTACTAGTGGTATCATGGAGGACTCCGCCATCAAATGCGAGTACATGCTTAA
  CGCCATCCCCAAGCGCCTGTGCCTCGTGTGCGGGGACATTGCCTCTGGCTACCACTACGGAGTGGCCTCC
  TGCGAGGCTTGCAAGGCGTTCTTCAAGAGAACCATTCAAGGCAACATCGAGTACAACTGCCCGGCCACCA
  ATGAATGTGAGATCACCAAACGGAGGCGCAAGTCCTGTCAGGCCTGCCGATTCATGAAATGCCTCAAAGT
  GGGGATGCTGAAGGAAGGTGTGCGCCTTGACCGAGTTCGAGGAGGCCGCCAGAAGTACAAGCGACGGCTG
  GATTCGGAGAACAGCCCCTACCTGAACCTGCCGATTTCCCCACCTGCTAAAAAGCCATTGACTAAGATCG
  TCTCGAATCTACTAGGGGTTGAGCAGGACAAGCTGTATGCTATGCCTCCCAACGATATCCCCGAGGGAGA
  TATCAAGGCCCTGACCACTCTCTGTGAATTGGCAGATCGGGAGCTTGTGTTCCTCATCAACTGGGCCAAG
  CACATCCCAGGCTTCCCCAGTCTGACACTTGGGGACCAGATGAGCCTGCTGCAGAGTGCCTGGATGGAGA
  TTCTCATCTTGGGCATCGTGTACCGCTCGCTCCCATACGATGACAAGCTGGCATACGCCGAGGACTATAT
  CATGGATGAGGAACACTCTCGCCTGGTAGGGCTGCTGGACCTTTACCGAGCCATCCTGCAGCTGGTGCGC
  AGGTACAAGAAACTCAAGGTAGAGAAGGAAGAGTTTATGATCCTCAAGGCCCTGGCCCTCGCCAACTCAG
  ATTCGATGTACATTGAGAACCTGGAGGCGGTGCAGAAGCTCCAGGACCTGCTGCACGAGGCGCTGCAGGA
  CTATGAGCTGAGTCAGCGCCACGAGGAGCCGCGGAGGGCCGGCAAGCTGCTGCTGACGCTGCCCCTGCTG
  AGGCAGACAGCCGCCAAAGCCGTGCAACACTTCTACAGTGTGAAACTGCAGGGCAAGGTGCCCATGCACA
  AACTCTTCCTGGAGATGCTGGAGGCCAAGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011934
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011934.4, NP_036064.3
• RefSeq Size: 4208 bp
• RefSeq ORF: 1365 bp
• Locus ID: 26380
• UniProt ID: Q61539
• Cytogenetics: 12 40.49 cM
• Gene Summary: Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcriptional activity (PubMed:27601327, PubMed:23169531, PubMed:23508100, PubMed:26206133, PubMed:20534447, PubMed:18662995, PubMed:18957414, PubMed:27723719, PubMed:23019124). Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways (PubMed:18957414, PubMed:26206133, PubMed:20534447, PubMed:23040478, PubMed:23040477, PubMed:23019124, PubMed:23169531). Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells (PubMed:26206133). Also regulates expression of multiple rod-specific genes and is required for survival of this cell type (PubMed:20534447). Plays a role as transcription factor activator of GATA6, NR0B1, POU5F1 and PERM1 (PubMed:18662995, PubMed:23508100, PubMed:18957414). Plays a role as transcription factor repressor of NFE2L2 transcriptional activity and ESR1 transcriptional activity (By similarity). During mitosis remains bound to a subset of interphase target genes, including pluripotency regulators, through the canonical ESRRB recognition (ERRE) sequence, leading to their transcriptional activation in early G1 phase (PubMed:27723719). Can coassemble on structured DNA elements with other transcription factors like SOX2, POU5F1, KDM1A and NCOA3 to trigger ESRRB-dependent gene activation (PubMed:23019124, PubMed:23169531, PubMed:18662995, PubMed:26206133). This mechanism, in the case of SOX2 corecruitment prevents the embryonic stem cells (ESCs) to epiblast stem cells (EpiSC) transition through positive regulation of NR0B1 that inhibits the EpiSC transcriptional program (PubMed:23169531). Also plays a role inner ear development by controlling expression of ion channels and transporters and in early placentation (PubMed:9285590, PubMed:17765677).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). CCDS Note: An upstream translation start codon is selected for this CCDS for consistency with other family members. The use of an alternative upstream start codon would result in a protein that is 21 aa longer at the N-terminal.