Tfap2b (NM_009334) Mouse Untagged Clone

CAT#: MC216130

Tfap2b (untagged) - Mouse transcription factor AP-2 beta (Tcfap2b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tfap2b
• Synonyms: AI606113; AP-2(beta); E130018K07Rik; Tcfap2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216130 representing NM_009334
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCACCTCCTAGAGACCAGGCTGCGATCATGCTCTGGAAACTCGTGGAGAATGTCAAGTACGAAG
  ACATCTATGAGGACCGGCACGATGGCGTCCCAAGCCATAGCTCGAGACTCTCTCAACTGGGCTCTGTGTC
  CCAAGGACCCTACTCAAGCGCCCCGCCGCTGTCCCACACGCCATCATCGGACTTCCAGCCACCCTACTTT
  CCGCCCCCCTACCAGCCGCTACCCTACCACCAGAGTCAAGACCCGTACTCCCACGTCAACGATCCCTACT
  CCCTGAACCCTCTGCATCAGCCCCAGCAGCACCCGTGGGGGCAACGGCAACGCCAAGAAGTGGGCTCAGA
  AGCCGGCTCTCTCCTGCCCCAGCCCCGGGCAGCCTTGCCCCAGCTCTCCGGCCTTGATCCCCGAAGGGAC
  TACCACTCTGTCCGCCGGCCGGACGTGCTGCTGCATTCCGCACATCACGGCCTGGACGCCGGCATGGGCG
  ACAGCCTCTCGTTGCACGGCCTTGGGCATCCCGGCATGGAAGACGTGCAGTCAGTTGAAGATGCCAATAA
  CAGCGGCATGAACCTATTGGACCAGTCAGTCATTAAAAAAGTTCCTGTCCCTCCCAAATCTGTGACTTCT
  CTAATGATGAATAAAGATGGCTTCTTGGGAGGAATGTCAGTCAACACCGGCGAGGTATTTTGCTCAGTCC
  CGGGCCGTTTATCTCTACTCAGTTCAACTTCAAAGTACAAAGTCACTGTGGGCGAAGTTCAGAGAAGGCT
  CTCGCCCCCTGAATGCCTCAATGCATCTCTCCTGGGCGGCGTCCTCAGAAGAGCCAAATCGAAAAATGGG
  GGGAGATCCTTGAGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGCAGGCGCAAAGCAGCAA
  ATGTCACGTTACTCACCTCACTGGTAGAAGGGGAAGCTGTTCACTTAGCTCGGGATTTCGGGTATATTTG
  TGAAACAGAGTTTCCTGCTAAAGCCGTGTCCGAGTATTTGAACAGACAGCACACGGACCCCAGTGACCTG
  CACTCCAGAAAGAATATGCTGCTGGCCACCAAGCAACTTTGTAAAGAATTTACGGATCTGCTGGCTCAGG
  ACCGGACACCGATCGGAAACAGCAGGCCCAGCCCCATCCTGGAGCCGGGCATCCAAAGCTGTCTCACGCA
  CTTCAGTCTCATCACGCACGGCTTCGGTGCCCCGGCCATTTGCGCTGCGCTCACGGCCCTGCAGAACTAT
  CTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACTAACAGGCACACGTCTGGGG
  AAGGCCCAGGTAGTAAAACTGGCGACAAGGAGGAGAAACACAGGAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009334
• Insert Size: 1380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009334.3, NP_033360.2
• RefSeq Size: 6132 bp
• RefSeq ORF: 1380 bp
• Locus ID: 21419
• UniProt ID: Q61313
• Cytogenetics: 1 A3
• Gene Summary: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.