Endou (NM_008902) Mouse Untagged Clone

CAT#: MC216128

Endou (untagged) - Mouse endonuclease, polyU-specific (Endou), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Endou
• Synonyms: Pp11r; Tcl-30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216128 representing NM_008902
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAACTGCTCTATTCATGTGGGGCAACTCAAGTTGGTTTTCAATCCAGAAGGGGCTCCCCTGTCTC
  TTCCTTTTTTTACATATTTTTCTTTTTATATCTTTTGGGTGCAGTGGGCACCAAATGCCAGAATGGTAGA
  AAATTTGTCACCTGGGGAAATTATTTTTGTTTTGTTTTGTTTAAGTTTTCCGTCATCTGACCTTGGAAAG
  CCTTCCATTTTTTCAGTCCTCAGCCTCCTGTGTCAGATTGGGGGCTGGGATGGGATGTGCGAATACCCTT
  TCAACACATGTGCTTGCCTCCAGGCTGAAGAAGGACCCCCAGAGCCAGAGGCATTCCTGGATCCAGAGGA
  CAAGATCCCAACCAGCCACCTCTACTCCGCACCGAGCTCCTGCCAGGGCCGCTGCCGGGAAGCCTACGAC
  AAGCACCACCCTTGCCACTGTAATGACCGCTGCCCAGAGTTTGGAAACTGCTGTGAGGATTTTGACAGTC
  TGTGTGGTGGTGATCATGAGGGTTTCTCCCACAGCAGCGATGCCGTCACTAAGGAGGAGCTTGAGAGCAT
  CTCCGAGACCATCTACAGGGTAGATACCAACAAAGCCCAGAAGGAAGACATTGTGCTCAACAGTCAGAAC
  CGGATCTCCCCATCAGAGACGGGGAACCAAGTGGATCGAAGCCCAGAGCCGCTCTTCACTTACGTCAACG
  AGAAGCTGTTCTCCAAGCCTACCTATGCAGCCTTTATCAACTTACTCAACAATTACCAGCGGGCCACAGG
  CCACGGGGAGCATTTCAGTGCCCAGCAACTGGAGGAGCAGGGTGTCTTCCTCAGGGAGGTCATGAAGACG
  GCTGTCATGAAGGAACTCTATAGCTTTCTCCATCACCAAAACCGTTACAGCTCAGAACAAGAGTTTGTGG
  ACGATTTGAAGAACATGTGGTTTGGGCTGTACTCGAGAGGCAATGATGAAGGGGACTCGAGTGGCTTTGA
  ACATGTCTTCTCTGGCGAAGTGAAGAAAGGCAAGGTCACTGGCTTTCATAACTGGATCCGCTTCTACCTG
  CAGGAGAAGGAGGGTCTGCTGGACTACTACAGTCACAACTACGACGGGCCCTGGGACTCCTACCCCGATG
  TCCTGGCCATGCAGTTCAATTGGGATGGCTACTACAAAGAAGTGGGCTCGGTCTTCATTGGCAGCAGCCC
  TGAGTTCGAGTTTGCCCTTTACTCTCTGTGCTTCATCACCAGGCCAGGCAAAAAGTGCCATCTGAGCCTG
  GGTGGCTATCCTCTGGCCATCCAGACCTACACCTGGGATAAGACCACCTACGGAAATGGCAAGAAGTACA
  TTGCCACAGCCTACGTGGTGTCTTCTTCCCAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008902
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008902.3, NP_032928.1
• RefSeq Size: 2404 bp
• RefSeq ORF: 1365 bp
• Locus ID: 19011
• UniProt ID: Q3V188
• Cytogenetics: 15 53.7 cM
• Gene Summary: Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.