Pde7a (NM_008802) Mouse Untagged Clone

CAT#: MC216126

Pde7a (untagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde7a
• Synonyms: AU015378; AW047537
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216126 representing NM_008802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAATCACTTTGATCTGGTGTCTGGCTTTGGTTCTGATCAAGTGGATCACCTCTAAGAGACGTGGAG
  CTATTTCCTATGACAGTTCTGATCAGACTGCGTTATATATTCGTATGCTAGGAGATGTCCGCGTGAGGAG
  CCGAGCAGGATTTGAAACAGAAAGAAGAGGTTCCCATCCGTACATTGACTTCCGTATTTTTCACTCTCAA
  TCTGACATTGAAGCATCAGTCTCCGCCAGGAACATCAGAAGGTTACTAAGTTTCCAGCGATATCTCAGAT
  CCTCACGAGTTTTTCGGGGTGCCACAGTTTGTAGTTCTCTAGACATTTTAGATGAGGATTACAATGGACA
  AGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTGTTTGATAGACTAACAAAT
  GGAAATAGTCTAGTAAGCCTAACCTTTCATTTATTTAGTCTTCATGGATTGATTGAGTACTTCCATTTAG
  ATATGGTGAAACTCCGTCGGTTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCACAA
  TGCAGTCCATGCTGCAGACGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAGTTCT
  GTCACTCCTTGGGATATCTTGCTGAGTTTAATTGCAGCCGCCACTCACGATCTGGATCACCCAGGTGTTA
  ATCAGCCGTTTCTTATTAAAACCAACCATTATCTAGCAACTTTATACAAGAATTCCTCAGTCCTGGAGAA
  TCACCACTGGAGATCTGCAGTGGGCTTGTTAAGAGAATCTGGTTTGTTCTCACACTTGCCATTGGAAAGC
  AGGCAAGAGATGGAGGCTCAGATAGGTGCTTTGATATTAGCCACGGATATCAGTCGCCAGAACGAGTACC
  TGTCATTGTTTAGATCTCACTTGGATAAAGGTGACTTACACCTTGACGATGGCAGACATAGGCATTTGGT
  TCTACAGATGGCCTTGAAATGTGCTGATATTTGTAACCCATGTCGGAACTGGGAATTAAGCAAGCAGTGG
  AGTGAAAAAGTAACGGAGGAATTCTTCCACCAAGGAGATATAGAAAAAAAGTACCATTTGGGTGTGAGTC
  CACTTTGTGATCGTCAGACTGAGTCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTAGTGGAGCC
  TTTATTTACAGAGTGGGCCAGGTTTTCAGACACACGGCTGTCACAGACGATGCTTGGACATGTGGGGCTG
  AATAAAGCCAGCTGGAAGGGACTGCAGAGACAACAGCCCAGCAGCGAGGATGCCAATGCTGCATTTGAGT
  TGAACTCACAGTTACTAACTCAGGAAAATCGGTTATCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008802
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008802.3, NP_032828.2
• RefSeq Size: 6028 bp
• RefSeq ORF: 1371 bp
• Locus ID: 18583
• UniProt ID: P70453
• Cytogenetics: 3 5.36 cM
• Gene Summary: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.