Il7r (NM_008372) Mouse Untagged Clone

CAT#: MC216118

Il7r (untagged) - Mouse interleukin 7 receptor (Il7r), (10ug)


  "NM_008372" in other vectors (6)

Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Il7r
Synonyms CD127; IL-7Ralpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216118 representing NM_008372
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCTCTGGGTAGAGCTTTCGCTATAGTTTTCTGCTTAATTCAAGCTGTTTCTGGAGAAAGTGGAA
ATGCCCAGGATGGAGACCTAGAAGATGCAGACGCGGACGATCACTCCTTCTGGTGCCACAGCCAGTTGGA
AGTGGATGGAAGTCAACATTTATTGACTTGTGCTTTTAATGACTCAGACATCAACACAGCTAATCTGGAA
TTTCAAATATGTGGGGCTCTTTTACGAGTGAAATGCCTAACTCTTAACAAGCTGCAAGATATATATTTTA
TAAAGACATCAGAATTCTTACTGATTGGTAGCAGCAATATATGTGTGAAGCTTGGACAAAAGAATTTAAC
TTGCAAAAATATGGCTATAAACACAATAGTTAAAGCCGAGGCTCCCTCTGACCTGAAAGTCGTTTATCGC
AAAGAAGCAAATGATTTTTTGGTGACATTTAATGCACCTCACTTGAAAAAGAAATATTTAAAAAAAGTAA
AGCATGATGTGGCCTACCGCCCAGCAAGGGGTGAAAGCAACTGGACGCATGTATCTTTATTCCACACAAG
AACAACAATCCCACAGAGAAAACTACGACCAAAAGCAATGTATGAAATCAAAGTCCGATCCATTCCCCAT
AACGATTACTTCAAAGGCTTCTGGAGCGAGTGGAGTCCAAGTTCTACCTTCGAAACTCCAGAACCCAAGA
ATCAAGGAGGATGGGATCCTGTCTTGCCAAGTGTCACCATTCTGAGTTTGTTCTCTGTGTTTTTGTTGGT
CATCTTAGCCCATGTGCTATGGAAAAAAAGGATTAAACCTGTCGTATGGCCTAGTCTCCCCGATCATAAG
AAAACTCTGGAACAACTATGTAAGAAGCCAAAAACGAGTCTGAATGTGAGTTTCAATCCCGAAAGTTTCC
TGGACTGCCAGATTCATGAGGTGAAAGGCGTTGAAGCCAGGGACGAGGTGGAAAGTTTTCTGCCCAATGA
TCTTCCTGCACAGCCAGAGGAGTTGGAGACACAGGGACACAGAGCCGCTGTACACAGTGCAAACCGCTCG
CCTGAGACTTCAGTCAGCCCACCAGAAACAGTTAGAAGAGAGTCACCCTTAAGATGCCTGGCTAGAAATC
TGAGTACCTGCAATGCCCCTCCACTCCTTTCCTCTAGGTCCCCTGACTACAGAGATGGTGACAGAAATAG
GCCTCCTGTGTATCAAGACTTGCTGCCAAACTCTGGAAACACAAATGTCCCTGTCCCTGTCCCTCAACCA
TTGCCTTTCCAGTCGGGAATCCTGATACCAGTTTCTCAGAGACAGCCCATCTCCACTTCCTCAGTACTGA
ATCAAGAAGAAGCGTATGTCACCATGTCTAGTTTTTACCAAAACAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008372
Insert Size 1380 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008372.4, NP_032398.3
RefSeq Size 3227 bp
RefSeq ORF 1380 bp
Locus ID 16197
UniProt ID P16872
Cytogenetics 15 4.16 cM
Gene Summary Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]

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