Htr2c (NM_008312) Mouse Untagged Clone

CAT#: MC216115

Htr2c (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Htr2c
• Synonyms: 5-HT2C; 5-HT2cR; 5-HTR2C; 5HT1c; Htr1; Htr1c; S; SR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216115 representing NM_008312
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACCTGGGCACTGCGGTGCGCTCACTCCTTGTGCACCTAATTGGCCTATTGGTTTGGCAGTTCG
  ATATTTCCATAAGTCCAGTAGCAGCTATAGTAACTGACACTTTTAATTCCTCCGATGGTGGACGCTTGTT
  TCAATTCCCGGACGGGGTACAAAACTGGCCAGCACTTTCAATAGTCGTGATTATAATCATGACAATAGGG
  GGCAACATTCTCGTTATCATGGCAGTAAGCATGGAGAAGAAACTGCACAATGCTACCAATTATTTCTTAA
  TGTCCCTAGCCATTGCTGATATGCTGGTGGGACTACTTGTCATGCCCCTGTCTCTGCTTGCAATTCTTTA
  TGATTATGTCTGGCCTTTACCTAGATATTTGTGCCCCGTCTGGATTTCACTAGATGTGCTATTTTCAACT
  GCGTCCATCATGCACCTCTGCGCCATATCGCTGGACCGGTATGTAGCAGTGCGTAATCCTGTTGAGCATA
  GCCGGTTCAATTCGCGGACTAAGGCCATCATGAAGATTGCCATCGTTTGGGCAATATCAATAGGAGTTTC
  AGTTCCTATCCCTGTGATTGGACTGAGGGACGAAAGCAAAGTGTTCGTGAATAATACTACCTGCGTGCTC
  AATGACCCGAACTTCGTTCTCATCGGGTCCTTCGTGGCATTCTTCATCCCGTTGACAATTATGGTGATCA
  CCTACTTCTTAACGATCTACGTCCTACGCCGTCAAACCCTGATGTTACTTCGAGGTCACACCGAGGAGGA
  ACTGCGTAATATCAGCCTGAACTTTCTAAAGTGCTGCTGCAAGAAGGGTGATGAGGAAGAGAACGCTCCC
  AACCCCAATCCAGATCAGAAGCCACGTCGAAAGAAGAAAGAAAAGCGGCCTAGAGGCACCATGCAAGCTA
  TCAACAATGAGAAGAAAGCTTCCAAAGTCCTTGGCATTGTATTCTTTGTGTTTCTGATCATGTGGTGCCC
  GTTTTTCATCACCAATATCCTGTCGGTGCTTTGTGGGAAGGCCTGTAACCAAAAGCTAATGGAGAAACTT
  CTCAATGTGTTTGTTTGGATTGGCTATGTGTGTTCAGGCATCAATCCTCTGGTGTACACTCTCTTCAACA
  AAATTTACCGAAGGGCTTTCTCTAAATATTTGCGCTGCGATTATAAGCCAGACAAAAAGCCTCCTGTTCG
  ACAGATTCCTAGGGTTGCTGCCACTGCTTTGTCTGGGAGGGAGCTCAATGTTAACATTTATCGGCATACC
  AATGAACGTGTAGTTAGGAAAGCTAATGACACAGAGCCTGGCATAGAGATGCAGGTAGAGAATTTAGAGC
  TGCCGGTCAATCCCTCTAATGTGGTCAGCGAGAGGATTAGTAGTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008312
• Insert Size: 1380 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC141085, AAI41086
• RefSeq Size: 3011 bp
• RefSeq ORF: 1380 bp
• Locus ID: 15560
• UniProt ID: P34968
• Cytogenetics: X 68.46 cM
• Gene Summary: Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008]