Chrm1 (NM_001112697) Mouse Untagged Clone

CAT#: MC216105

Chrm1 (untagged) - Mouse cholinergic receptor, muscarinic 1, CNS (Chrm1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrm1
• Synonyms: Chrm-1; M1; M1R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216105 representing NM_001112697
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACCTCAGTGCCCCCTGCTGTCAGTCCCAACATCACCGTCTTGGCACCAGGAAAGGGTCCCTGGC
  AAGTGGCATTCATCGGGATCACCACAGGCCTCCTGTCTCTGGCTACAGTGACAGGCAACCTGCTGGTGCT
  CATCTCCTTCAAGGTCAACACAGAGCTCAAGACAGTCAACAACTACTTCCTGCTGAGCCTGGCCTGTGCT
  GATCTCATCATTGGCACTTTCTCCATGAACCTCTATACCACATACCTGCTCATGGGCCACTGGGCTCTGG
  GCACACTGGCCTGTGACCTCTGGCTGGCCCTGGACTATGTGGCCAGCAACGCCTCTGTCATGAATCTTCT
  GCTCATCAGCTTTGACCGTTACTTCTCAGTGACCCGACCCCTGAGCTACCGAGCCAAGCGCACTCCCCGC
  AGGGCAGCTCTGATGATTGGCCTCGCGTGGTTGGTTTCCTTCGTTCTCTGGGCCCCAGCCATCCTCTTCT
  GGCAATACCTAGTTGGGGAGCGGACAGTGCTGGCTGGGCAGTGCTACATCCAGTTCCTCTCCCAACCCAT
  CATCACTTTTGGCACAGCCATGGCCGCCTTCTACCTCCCTGTCACAGTCATGTGTACGCTGTACTGGCGC
  ATCTACCGGGAGACAGAAAACCGAGCCCGGGAGCTAGCAGCCCTACAGGGCTCTGAGACACCAGGCAAAG
  GTGGTGGCAGCAGCAGCAGCTCAGAGAGGTCACAGCCAGGAGCTGAAGGCTCACCCGAGTCACCTCCAGG
  CCGCTGCTGTCGCTGTTGCCGGGCACCCAGACTTCTGCAGGCCTACAGCTGGAAAGAAGAAGAGGAAGAG
  GATGAAGGCTCCATGGAGTCCCTCACATCCTCTGAAGGTGAGGAGCCTGGCTCAGAAGTGGTGATCAAGA
  TGCCTATGGTAGATCCTGAGGCACAGGCACCCACCAAGCAGCCTCCCAAAAGCTCCCCAAATACAGTCAA
  GAGGCCCACCAAGAAAGGCCGAGACCGAGGCGGCAAAGGCCAAAAACCCCGAGGGAAGGAACAACTGGCC
  AAGAGAAAGACCTTCTCACTGGTCAAGGAGAAGAAGGCAGCTCGGACCCTGAGTGCCATCCTGCTGGCCT
  TCATCCTCACCTGGACACCATATAACATCATGGTGCTGGTGTCTACATTCTGCAAGGACTGTGTTCCAGA
  AACCCTATGGGAGCTGGGCTACTGGCTTTGCTACGTCAACAGCACTGTCAACCCCATGTGCTACGCACTC
  TGCAACAAAGCCTTCCGGGACACTTTCCGCCTGCTGTTGCTCTGCCGCTGGGACAAGCGGCGCTGGCGCA
  AAATCCCCAAGCGCCCTGGCTCTGTGCACCGCACCCCCTCCCGCCAATGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001112697
• Insert Size: 1383 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112697.1, NP_001106167.1
• RefSeq Size: 6301 bp
• RefSeq ORF: 1383 bp
• Locus ID: 12669
• UniProt ID: P12657
• Cytogenetics: 19 A
• Gene Summary: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.