Amy2b (NM_001190403) Mouse Untagged Clone

CAT#: MC216076

Amy2b (untagged) - Mouse amylase 2b (Amy2b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Amy2b
• Synonyms: Amy-X; Amy2-2; mAmy2-1; OTTMUSG00000022462
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216076 representing NM_001190403
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTCAGATG
  GGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGATACTT
  AGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAATATTGTAATTCATAACCCATCA
  AGGCCTTGGTGGGAAAGATATCAACCAATCAGCTACAAAATTTGCACAAGGTCTGGAAATGAAGATGAAT
  TCAGAGACATGGTGACAAGGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACAT
  GTGTGGCTCAGGCAATCCTGCAGGAACAAGCAGTACCTGTGGAAGTTACCTCAATCCAAATAACAGGGAA
  TTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATTAGTAACTACA
  ATGATGCTTATCAGGTCAGAAATTGTCGTCTGTCTGGCCTTCTGGATCTTGCACTTGAGAAAGATTATGT
  TCGTACCAAGGTTATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTA
  ACAGAATTCAAGTTTGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATGGAGAGAAGATGTCCTATT
  TAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGACAGAGCCCTTGTGTTTGTGGACAACCATGA
  CAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCTGACATTCTGGGATGCTAGAATGTACAAAATG
  GCTGTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAATAGAA
  ATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCTAATAACAATGGAGTAACAAAAGAAGT
  GACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACATAGATGGCGTCAAATAAGGAAC
  ATGGTTGCCTTCAGGAATGTAGTCAATGGTCAGCCTTTTGCAAACTGGTGGGATAATAACAGCAACCAAG
  TAGCTTTTAGCAGAGGAAACAGAGGCTTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTT
  ACAGACTGGTCTTCCTGCTGGCACATACTGTGATGTCATCTCTGGAGATAAGGTCGATGGCAATTGCACT
  GGACTTAAAGTAAATGTTGGCAGTGATGGTAAAGCTCACTTTTCCATTAGTAACTCTGCTGAGGACCCAT
  TTATTGCAATCCATGCTGACTCAAAACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001190403
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190403.1, NP_001177332.1
• RefSeq Size: 1413 bp
• RefSeq ORF: 1362 bp
• Locus ID: 545562
• Cytogenetics: 3 49.35 cM
• Gene Summary: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The mouse genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. At least one mouse strain has a non-functional allele of this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.