Tubb1 (NM_001080971) Mouse Untagged Clone

SKU
MC216075
Tubb1 (untagged) - Mouse tubulin, beta 1 (Tubb1), (10ug)
  $732.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tubb1
Synonyms 2810484G07Rik; M(beta)1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216075 representing NM_001080971
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGAGATCGTGCACATCCAGATTGGCCAGTGTGGCAACCAGATCGGAGCCAAGTTCTGGGAGGTGA
TCGGGGAAGAACATGGGATTGACTGCGCTGGGAGCTACTGTGGGACGTCTGCTCTCCAGCTGGAGAGGAT
CAGCGTTTACTACAACGAAGCCTACGGTAAGAAGTATGTGCCGCGAGCCGTCCTTGTGGACCTGGAACCC
GGGACAATGGACAGCATCCGATCCAGCAGATTGGGAGTCCTCTTTCAGCCTGACAGTTTTGTTCATGGTA
ACTCTGGTGCTGGTAACAACTGGGCGAAGGGCCACTACACAGAGGGAGCGGAGCTGATCGAAAATGTCAT
GGATGTGGTAAGGAGGGAGAGCGAGAGCTGCGACTGCCTACAGGGTTTCCAGATCGTGCATTCTCTGGGT
GGAGGCACGGGCTCAGGCATGGGCACCCTGCTCATGAACAAGATCAGGGAGGAGTACCCGGATCGGATCT
TGAACTCCTTTAGCGTCATGCCATCACCCAAGGTATCAGACACGGTGGTAGAGCCCTACAATGCCGTGCT
ATCCATCCACCAGCTGATAGAGAACACAGATGCCTGCTTCTGCATCGACAACGAAGCGCTCTATGACATT
TGTTTCCGCACCCTGAGGCTGACGACACCCACCTACGGGGATCTCAACCACCTGGTGTCCTTGACCATGA
GCGGAATCACGACTTCACTGCGCTTTCCCGGTCAGCTCAATGCAGACCTGCGCAAGCTGGCCGTGAATAT
GGTGCCCTTTCCTCGTCTTCACTTCTTCATGCCTGGCTTCGCTCCACTCACAGCCCAGGGTAGCCAGCAG
TACCGAGCCCTCTCAGTAGCGGAGCTCACCCAACAGATGTTCGATGCCCGCAACATCATGGCTGCCTGTG
ACCCTCGCCGCGGCCGCTACCTGACTGTGGCTTGTATATTCCGGGGTAAGATGTCCACCAAGGAAGTGGA
CCAACAGCTGCTGTCCATTCAGACAAGGAACAGCAACTGCTTTGTGGAATGGATTCCCAACAATGTCAAG
GTAGCTGTGTGTGACATCCCGCCCCGTGGGCTGAACATGGCAGCTACCTTCCTGGGCAACAATACAGCCA
TCCAAGAGCTCTTCACCAGGGTCTCTGAGCACTTCTCAGCTATGTTCAGAAGGAGAGCTTTTGTGCACTG
GTACACCAGTGAAGGGATGGACATAAGTGAATTTGGGGAAGCTGAGAGTGATATCCATGACCTGGTGTCT
GAGTACCAACAATTCCAAGATGTCAGAGCAGGGCTAGAGGACAGTGAAGAGGATGTGGAGGAGGCAGAGG
TGGAGGCAGAAGACAAAGATCATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001080971
Insert Size 1356 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080971.2, NP_001074440.1
RefSeq Size 2004 bp
RefSeq ORF 1356 bp
Locus ID 545486
UniProt ID A2AQ07
Cytogenetics 2 H4
Summary Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001080971" in other vectors (4)
SKU Description Size Price
MG224088 Tubb1 (tGFP-tagged) - Mouse tubulin beta 1 (Tubb1), (10ug) 10 ug
$886.00
MR224088 Tubb1 (Myc-DDK-tagged) - Mouse tubulin, beta 1 (Tubb1) 10 ug
$686.00
MR224088L3 Lenti ORF clone of Tubb1 (Myc-DDK-tagged) - Mouse tubulin, beta 1 (Tubb1) 10 ug
$986.00
MR224088L4 Lenti ORF clone of Tubb1 (mGFP-tagged) - Mouse tubulin, beta 1 (Tubb1) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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