Alg11 (NM_183142) Mouse Untagged Clone

CAT#: MC216034

Alg11 (untagged) - Mouse asparagine-linked glycosylation 11 homolog (yeast, alpha-1,2-mannosyltransferase) (Alg11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alg11
• Synonyms: AI849156; AW492253; B230397C21; Mmat-4(5)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216034 representing NM_183142
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCTGCGGTTTTTCTATTCACTCTTCTTTCCTG
  GACTGATGATATGCGGAGTTTTGTGTGTATATTTGGTCATTGGCCTTTGGGTTATCAGGTGGCATCTACA
  GAGAAAGAAGAAGTCTGTGTCGACCAGCAAAAATGGAAAAGAACAAACGGTGGTTGCATTTTTCCATCCA
  TACTGTAATGCAGGTGGCGGAGGAGAGAGAGTTCTGTGGTGTGCTTTGAGAGCCCTGCAGAAAAAGAAAC
  GCTACCTTGTGGAAGATTCCCGGTATCCTCACTTCACATTGCTAGGCCAAAGTCTTGGATCCATTCTTCT
  CGGCTGGGAAGCTCTGATGCAGCGCGTTCCTGATGTTTACATCGACTCAATGGGATACGCCTTCACACTC
  CCTCTCTTTAAGTATGTGGGAGGTTGCCGAGTCGGAAGCTATGTTCACTATCCCACGATCAGCACTGACA
  TGCTGTCTGTGGTGAAGAATCAGAATCCTGGATTTAATAATGCTGCTTTCATTAGCAGAAATGCTCTTCT
  CAGCAAAGCAAAGCTCATCTATTACTATTTATTTGCTTTTGTTTATGGCCTTGTTGGATCTTGCAGTGAC
  ATAGTCATGGTTAATTCTTCTTGGACATTAAACCATATCCTCTCACTGTGGAAGGTTGGGCATTGCACTA
  ACATTGTCTATCCACCTTGTGATGTGCAGACATTTCTGGACATCCCTTTACATGAGAAGAAGGTGACCCC
  AGGGCATTTGCTGGTTTCCATTGGCCAGTTCAGACCTGAAAAGAATCATGCTTTGCAGATCAAAGCCTTT
  GCTAAATTGTTAAATGAGAAAGCAGCTGAGTTAGGCCATTCTCTTAAACTTGTCCTTATTGGAGGGTGTC
  GCAACAAAGATGATGAGTTTAGAGTAAACCAACTGAGAAGTCTATCTGAGAATTTGGGAGTTCAAGAAAA
  TGTGGAATTTAAAATAAACATTTCATTCGATGAATTGAAGAATTACTTGTCTGAAGCGACCATCGGTCTG
  CATACTATGTGGAATGAGCATTTTGGGATTGGAGTTGTCGAGTGTATGGCAGCTGGCACAGTTATCCTTG
  CACACAACTCAGGAGGCCCGAAGCTGGACATTGTCATTCCTCATGAAGGACAGATAACTGGCTTTCTGGC
  TGAGAGTGAAGAAGGCTATGCTGACTCTATGGCTCACATTCTTTCTCTGTCTGCAGAAGAGAGACTCCAA
  ATCAGGAAGAATGCTAGAGCGTCTATAAGCAGATTCTCCGACCAGGAGTTTGAAGTAGCATTCCTGTGTT
  CCATGGAGAAGTTACTTACTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183142
• Insert Size: 1353 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183142.4, NP_898965.1
• RefSeq Size: 4844 bp
• RefSeq ORF: 1353 bp
• Locus ID: 207958
• UniProt ID: Q3TZM9
• Cytogenetics: 8 A2
• Gene Summary: Mannosyltransferase involved in the last steps of the synthesis of Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum. Catalyzes the addition of the 4th and 5th mannose residues to the dolichol-linked oligosaccharide chain (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.