Acot2 (NM_134188) Mouse Untagged Clone

CAT#: MC216033

Acot2 (untagged) - Mouse acyl-CoA thioesterase 2 (Acot2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acot2
• Synonyms: AA571646; MTE-I; Mte1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216033 representing NM_134188
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCTCGTCTTTCGCTGTCCTGAGAGCAAGCAGGTTGTGCCAACAGGATTGGAAGAGCTGGGCGC
  GGCTGTTTGTACCTCCGCCGCTCAGCACTGGTGGCCGGACCACTTGGGCGCGGACAAATGCGACGCTGAG
  CGTAGAGCCCGAGGGCCGCAGCTGCTGGGACGAGCCGCTGAGCATCGCCGTGCGCGGCCTGGCCCCCGAG
  CAGCCCGTCACGCTGCGCTCTGCCTTGCGCGACGAGAAGGGCGCGCTCTTCCGAGCCCACGCGCGCTACC
  GCGCCGACGCTGGCGGCGAGCTGAACCTGGCGCGCGCGCCCGCGCTGGGCGGCAGCTTCTCGGGGCTCGA
  GCCCATGGGGCTGCTCTGGGCCATGGAGCCCGAACGGCCTCTCTGGCGCCTGATCAAGCGCGACGTGCAG
  ACGCCCTTCTTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGATGGCGGGCAGCGGCTGGCGCAGG
  CGGTGCACGAGCGTCACTTCTTGGCTCCCGGGGTGCGGCGCGTGCCCGTACGAGAGGGCCGGGTGCGCGC
  CACGCTCTTCCTGCCCCCAGAACCTGGACCCTTTCCTGGGATCATAGACCTTTTTGGAGTTGGAGGTGGC
  CTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTGATGGCTCTGGCTTATTACAACT
  ACGACGACCTCCCCAAGAGCATAGAAACCATGCACATGGAGTACTTTGAAGAAGCCGTGAACTACCTGCG
  CAGCCACCCCGAGGTAAAAGGACCTGGAATTGGGCTGCTTGGGATTTCCAAAGGGGGTGAACTTGGCCTT
  GCTATGGCCTCCTTCCTGAAGGGCATCACAGCTGCTGTGGTCATCAATGGCTCCGTGGCTGCTGTTGGGA
  ACACCATCTCCTACAAGGATGAGACTATACCCCCTGTGTCCCTTCTGAGAAACCAAGTCAAAATGACCAA
  AGATGGTCTCTTGGATGTCGTGGAAGCTCTGCAAAGCCCTCTGGTAGACAAGAAGAGCTTCATTCCCGTG
  GAAAGGTCTGACACGACCTTCCTGTTCCTCGTAGGTCAGGACGACCACAACTGGAAGAGCGAGTTCTATG
  CTGATGAGATCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCCCAGATCATCTGCTACCCAGCAGC
  AGGGCATTACATCGAGCCCCCTTACTTCCCGCTGTGCAGCGCTGGCATGCACCTCCTGGTGGGTGCCAAC
  ATCACCTTTGGAGGGGAGCCCAGGGCTCATGCCGTGGCCCAGGTGGACGCATGGCAGCAGCTCCAGACTT
  TCTTCCACAAACAGTTGGGTAGCAAGAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134188
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134188.3, NP_598949.3
• RefSeq Size: 2215 bp
• RefSeq ORF: 1362 bp
• Locus ID: 171210
• UniProt ID: Q9QYR9
• Cytogenetics: 12 D1
• Gene Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:25114170). Acyl-coenzyme A thioesterase 2/ACOT2 displays higher activity toward long chain acyl CoAs (C14-C20) (PubMed:25114170). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria (PubMed:25114170).[UniProtKB/Swiss-Prot Function]