Tmprss3 (NM_080727) Mouse Untagged Clone

CAT#: MC216032

Tmprss3 (untagged) - Mouse transmembrane protease, serine 3 (Tmprss3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmprss3
• Synonyms: MGC130589
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216032 representing NM_080727
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGAATGACCCTCCGGCAGCCGAAGCTCCCTTCTCCTTCCGGTCTCTCTTCGGCTTGGATGATT
  TGAAAATAAGTCCTGTGGCACCAGATGGAGACGCAGTGGCTGCGCAGATCTTGTCCCTTCTGCCCTTGAA
  GTTTTTTCCGATCATCGTCATTGGGATCATCGCCTTGATACTGGCGCTGGCCATCGGTCTGGGCATCCAC
  TTCGACTGCTCTGGGAAGTACAGGTGCCATTCATCTTTCAAGTGTATTGAACTAACAGCTCGATGCGACG
  GGGTCTCCGACTGCAAGAACGCAGAGGATGAGTACCGATGCGTGCGGGTGAGCGGCCAAAGAGCGGCGCT
  CCAAGTGTTCACAGCTGCTGCGTGGAGGACCATGTGCTCCGATGACTGGAAAAGCCACTATGCCAAGATT
  GCCTGTGCCCAGCTGGGGTTTCCCAGCTATGTGAGCTCAGACCACCTAAGAGTGGATGCGCTGGAGGAGC
  AGTTCCAGGGCGACTTCGTGTCCATCAATCACCTCTTGTCAGATGACAAGGTGACGGCGTTACACCACTC
  TGTGTACATGAGGGAAGGCTGCACCTCAGGCCACGTGGTCACCTTGAAGTGCTCAGCCTGTGGCACGAGA
  ACGGGCTACAGTCCCCGAATTGTGGGTGGAAACATGTCCTCGCTCACTCAGTGGCCCTGGCAGGTCAGCC
  TCCAGTTCCAGGGGTACCACCTATGTGGGGGCTCCATCATCACCCCTCTGTGGATCGTCACTGCGGCACA
  CTGTGTTTATGACCTGTACCACCCCAAGTCCTGGACTGTCCAGGTGGGTCTTGTGTCCCTGATGGACAGT
  CCTGTGCCCTCCCATCTGGTGGAGAAAATCATCTATCACAGCAAGTACAAGCCAAAGCGGCTGGGCAATG
  ACATAGCCCTCATGAAGCTGTCCGAGCCACTCACCTTTGACGAGACCATCCAGCCCATCTGTCTGCCCAA
  CTCTGAAGAGAACTTTCCCGATGGGAAACTGTGCTGGACCTCAGGATGGGGAGCCACAGAGGATGGAGGT
  GACGCCTCCCCTGTCCTGAACCACGCGGCTGTCCCTTTAATTTCAAACAAGATCTGCAACCACAGGGACG
  TGTATGGTGGTATCATCTCTCCCTCCATGCTCTGTGCAGGCTACCTGAAGGGCGGTGTGGACAGCTGCCA
  GGGAGACAGTGGAGGACCCCTGGTGTGCCAGGAGAGGAGACTGTGGAAGTTAGTGGGTGCAACAAGTTTT
  GGAATCGGCTGTGCTGAAGTGAACAAGCCTGGAGTCTATACGCGAATCACCTCCTTCCTGGACTGGATTC
  ACGAACAGTTGGAGAGAGATCTGAAGACTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080727
• Insert Size: 1362 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080727.2, NP_542765.2
• RefSeq Size: 2738 bp
• RefSeq ORF: 1362 bp
• Locus ID: 140765
• UniProt ID: Q8K1T0
• Cytogenetics: 17 A3.3
• Gene Summary: Probable serine protease that plays a role in hearing. Acts as a permissive factor for cochlear hair cell survival and activation at the onset of hearing and is required for saccular hair cell survival. Activates ENaC (in vitro).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.