Eif2b3 (NM_001111277) Mouse Untagged Clone

CAT#: MC216029

Eif2b3 (untagged) - Mouse eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2b3
• Synonyms: 1190002P15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216029 representing NM_001111277
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTTCAAGCGGTGGTTATGGCCGTTGGTGGGGGATCTCGGATGACAGACTTGACTTCCAGCATTC
  CCAAGCCTCTGCTTCCAGTTGGAAACAAACCTTTAATTTGGTACCCGCTGAACCTGCTTGAGCGTGTTGG
  ATTTGAAGAAGTCATCGTGGTTACAACCAAAGATGTCCAAAAGGCTCTCTGCGCAGAATTCAAGATGAAG
  ATGAAGCTAGATATTGTGTGCATTCCTGATGAGGCAGACATGGGGACTGCAGATTCTCTACGACACATCT
  ATCCAAAGCTTAAGACCGATGTGCTGGTCCTGAGCTGCGATCTGATAACAGATGTGGCTTTACATGAGGT
  TGTGGACCTGTTCAGAGCTTATGATGCGTCACTGGCCATGCTAATGAGGAAAGGACAAGAGAGCATAGAA
  CCAGTTCCAGGTCAAAAGGGGAAAAAAAAACCAGTGGAGCAGCGTGACTTCATTGGAGTGGACAGCACAG
  GAAAAAGGCTGCTCTTTATGGCTAATGAAGCAGACTTGGATGAAGAGCTGGTCATTAAGGGATCCATCCT
  GCAGAAGCACCCTAGGATACATTTCCACACAGGCCTTGTGGATGCCCATCTCTACTGCCTGAAGAAGTAC
  GTTGTGGATTTCTTAATGGAGAATAGGTCAATCACTTCTATCCGAAGCGAACTGATTCCATACCTAGTAA
  GAAAACAGTTTTCCTCAGCTTCCTCACAGCAAAGGCAGGAAGACAAAGAAGAGGATCTAAAGAAAAAGGA
  GCTGAAGTCTTTAGATATTTACAGTTTTATAAAAAAAGACGACACTCTGACCTTGGCTCCCTATGATGCC
  TGCTGGAATGCTTTTCGAGGAGACAAATGGGAAGATTTGTCTAGATCACAGGTCCGCTGCTATGTCCACA
  TCATGAAAGAAGGACTTTGCTCTCGAGTAAGCACACTGGGACTCTACATGGAAGCCAACAGACAGGTCCC
  CAAATTGCTATCTGTTCTTTGTCCGGAGGAATCAATGATCCATTCATCAGCCCAGATTGTCAACAAACAC
  CTGATTGGAGCTGATAGCCTCATCGGGCCAGATACACAAATTGGAGAGAAGTCATCTATTAAGCACTCTG
  TCATTGGCTCATCCTGTGTCATACGAGACAGAACGTCTATTACCAATTGCCTCCTCATGAACTCTGTCAC
  CGTGGAAGAAGGGAGCAGCATCCACGGCAGTGTCATCTGCAACAACGCAGTGATCGAGACGGGCGCAGAG
  ATCAAGAACTGTTTGGTTGGAAGTGGTCAGAGGATTGAAGCCAAAGCTAAACGAATGAACGAGGTGATCG
  TGGGGAATGACCAGCTCATGGAAATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001111277
• Insert Size: 1359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111277.1, NP_001104747.1
• RefSeq Size: 1565 bp
• RefSeq ORF: 1359 bp
• Locus ID: 108067
• Cytogenetics: 4 D1