Atf7ip2 (NM_153123) Mouse Untagged Clone

CAT#: MC216015

Atf7ip2 (untagged) - Mouse activating transcription factor 7 interacting protein 2 (Atf7ip2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atf7ip2
• Synonyms: 4930558K11Rik; BC018510; Get-1; PSM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216015 representing NM_153123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGTCCAGACAGAAAAAGACAGAAGGTTTTAAAGGCCAAGAAAACAATGCCTACAAGTTACCAGA
  AGCAGTTGGAGATCCTGAACAAGTCAACGAATGTTGAAGCTCCAAAAACAACAGTTGGAACTAATATTCC
  AAATGGTCATAATCAGAAGATGTTTTCAAAAAACAAAGAAAATGTTAAGGTCATGAAAGTTTCAGAGCAA
  ATTAATGAAAATGCTTGTGGGGCTCTGGAAAGACATACAGCACTGCTAGAACAGGTTAAACATTGGATTC
  GACAGGAGATTTGCATGATAAACTGTAATTTATTTGATAAAAAACTGAATGAATTGAATGAACGCATTGG
  GAAGACCCAGTGCAAGAGTAGACATGAAGCAATAGCTGGTGAACTCTTTGTAAAAATAAGAAGACTTCAA
  AAACGCATCAAAACAGTATTATCATCTCAAAGAAATTGTTTGGAACCAAACACATTACCCAGTAATACAG
  TCTGTAAGGTTACAGATTCAGAGGCTATGAATTTGAATGTGACCCAAAAGTCAGTTAAAAGTCGAAGTAA
  AAGAATATCTTCTGTGAACCATACCCCTTTAAACTCTTCAGAAAAAGCAGGTAGAAAGACTAATTTGCCA
  TCAACTTGTGTTGAGTTTGCTTCTGAAAGTAACACTGATGACGTTATGTTGATTTCTGTGAAAAATTCTA
  ATTTGACAACTTCAATTACATCAGAGCAAACAGAAATTAGAAAAAATACATCAAGAAATTTGAGCAACTC
  ACCTAATAGCATGATTAAAGTTGGGCCCGTAGAGAAGAAATTTGATTTTGTTATTGACTTGACAAGAGAA
  GGCCCGTCCAACTACAGCATAGAAAGCCCATCTTTCACCCTGAAGTCAACTTCGAAAGCTGTTTTAAGAT
  CAAAAGAAATAATTCCAGTGGCAGAAAATGGAAATGAGGGTTTTGGTTCATTTGAACACCTTCCACCTCT
  CCCAGAGCCACCAGCACCACTCCCTGAGATGGCAGACAAAATCAAAGATACACTTCCTCCCCAAAAGCCT
  GAGCTGAAAGTGAAGTGGGTGCTGAGGCCCACAAGCATTGCCCTGACATGGAACATCCCCAAGGTCAACC
  CCAACTGTGCGCCTGTGGAGAGTTACCACCTCTTCCTGTACTATGAGAACTCTGATCACTTGACGTGGAA
  GAAAATTGCAGAAATTAAAGCTTTACCACTTCCCATGGCTTGCACCTTATCTCAGAATTTAGCTTCTACC
  AAGTATTATTTTGCTGTCCAGTCAAAAGACATTTTTGGGAGATATGGACCATTTTGTAATATCAAATCTA
  TTCCTAGGTTTTCTGAAAACCTTACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153123
• Insert Size: 1359 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153123.2, NP_694763.2
• RefSeq Size: 2584 bp
• RefSeq ORF: 1359 bp
• Locus ID: 75329
• UniProt ID: Q3UL97
• Cytogenetics: 16 A1
• Gene Summary: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.