Tubb2b (NM_023716) Mouse Untagged Clone

CAT#: MC216011

Tubb2b (untagged) - Mouse tubulin, beta 2B (Tubb2b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tubb2b
• Synonyms: 2410129E14Rik; brdp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216011 representing NM_023716
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGAGATCGTGCACATTCAGGCGGGCCAGTGCGGCAACCAGATCGGTGCCAAGTTTTGGGAGGTCA
  TCAGTGATGAGCATGGTATAGACCCCACTGGAAGTTACCATGGAGACAGTGATTTGCAACTGGAAAGAAT
  CAATGTATACTACAATGAAGCAACTGGTAATAAATATGTGCCTAGGGCCATCCTGGTGGACCTGGAGCCA
  GGCACAATGGACTCAGTCAGGTCTGGACCATTTGGGCAGATCTTCAGGCCGGACAACTTCGTGTTCGGCC
  AGAGTGGTGCAGGAAATAACTGGGCAAAGGGCCACTACACAGAGGGGGCCGAGCTGGTGGACTCTGTCCT
  GGACGTGGTGAGGAAGGAGTCAGAAAGCTGTGACTGTCTTCAGGGCTTCCAGCTGACCCACTCACTGGGG
  GGAGGCACTGGCTCAGGCATGGGGACCCTGCTCATCAGCAAGATCAGAGAGGAGTACCCAGACCGCATCA
  TGAACACCTTCAGCGTCATGCCCTCACCCAAGGTCTCTGACACTGTGGTGGAGCCCTATAATGCCACCCT
  CTCAGTGCACCAGCTGGTAGAGAACACAGATGAAACCTACTGCATCGATAATGAGGCTCTGTATGACATC
  TGTTTCCGCACCCTGAAGCTGACCACACCCACCTATGGCGATCTCAACCACCTGGTGTCAGCCACCATGA
  GTGGAGTGACCACCTGCCTGCGCTTCCCAGGCCAGCTGAACGCAGACCTGCGCAAGCTGGCCGTGAACAT
  GGTGCCATTCCCACGCCTGCACTTCTTCATGCCAGGCTTTGCACCTCTGACCAGCCGGGGCAGCCAGCAG
  TACCGAGCCCTGACGGTGCCCGAGCTGACCCAGCAGATGTTCGACTCCAAGAACATGATGGCTGCCTGCG
  ACCCTCGCCATGGCCGCTACCTGACCGTGGCCGCCATTTTCCGTGGCCGCATGTCCATGAAGGAGGTGGA
  TGAGCAGATGCTCAACGTGCAGAACAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTCAAG
  ACGGCCGTGTGTGACATCCCTCCTCGTGGCCTCAAGATGTCAGCCACCTTCATTGGCAACAGCACTGCCA
  TCCAGGAGCTGTTCAAGCGCATCTCGGAGCAGTTCACTGCTATGTTCCGGCGCAAGGCTTTCCTGCACTG
  GTACACGGGTGAGGGCATGGACGAGATGGAGTTCACCGAGGCGGAGAGCAACATGAATGACCTGGTGTCT
  GAGTACCAGCAGTACCAGGATGCCACGGCTGATGAGCAGGGCGAGTTCGAGGAGGAAGAGGGCGAGGATG
  AGGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023716
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023716.2, NP_076205.1
• RefSeq Size: 1922 bp
• RefSeq ORF: 1338 bp
• Locus ID: 73710
• UniProt ID: Q9CWF2
• Cytogenetics: 13 A3.3
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Plays a critical role in proper axon guidance in both central and peripheral axon tracts. Implicated in neuronal migration.[UniProtKB/Swiss-Prot Function]