Gorasp2 (NM_027352) Mouse Untagged Clone

CAT#: MC216005

Gorasp2 (untagged) - Mouse golgi reassembly stacking protein 2 (Gorasp2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gorasp2
• Synonyms: 0610011A07Rik; 2410043M02Rik; 5730520M13Rik; 9430094F20Rik; AW552058
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216005 representing NM_027352
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCTCGCAGAGCGTCGAGATCCCGGGCGGCGGCACCGAGGGCTACCACGTTTTGCGGGTACAGG
  AAAACTCCCCAGGACACAGAGCTGGACTGGAGCCTTTCTTTGATTTTATTGTTTCTATTAACGGTTCAAG
  ATTAAATAAAGACAATGACACTCTGAAGGATCTGCTGAAAGCCAACGTTGAGAAGCCTGTAAAAATGCTC
  ATCTACAGCAGCAAAACCCTGGAGCTGCGGGAGGCCTCGGTCACACCGAGCAACTTGTGGGGCGGCCAGG
  GCTTGTTGGGGGTTAGCATTCGTTTCTGCAGCTTTGACGGGGCCAACGAAAACGTTTGGCATGTGCTGGA
  AGTGGAATCCAATTCCCCAGCAGCGCTGGCAGGATTGAGACCTCACAGTGATTACATCATTGGAGCTGAT
  ACCGTCATGAATGAGTCTGAAGACCTGTTCAGCCTTATTGAGACCCACGAAGCAAAGCCCCTGAAGCTCT
  ACGTGTACAACACAGACACAGACAACTGCAGAGAAGTGATCATCACACCAAACTCTGCATGGGGCGGAGA
  AGGCAGCCTTGGATGTGGCATTGGCTATGGTTATTTGCACCGAATACCTACACGCCCCTTTGAAGAAGGA
  AAGAAGATTTCCCTTCCAGGACAAATGACTGGTACCCCCATTACTCCTCTTAAGGATGGGTTTACAGAGG
  TCCAGCTGTCTTCAGTCAGTCCTCCATCTTTGTCACCACCAGGAACTACAGGAGTTGAGCAGAGTCTGTC
  TGGACTCTCCATTAGCTCGGCACCACCAGCTGTCAGCAATGTTCTCAGTACAGGCGTACCAACTGTACCA
  TTGTTGCCGCCACAAGTAAACCAGTCCCTTGCTTCTATGCCACCAATGAACCCAGCTACCACATTACCAA
  GTCTGATGCCTTTGTCAGCAGGGCTGCCTAGCCTCCCCAACCTCCCCAGCCTCTCCAACTTCAACCTCCC
  TGCTCCGCACATCATGCCAGGGGTCGGTTTGCCAGAGCTCGGGAGCCCGGGTTTGCCACCTCTTCCCTCC
  TTGCCTCCCCGAAACTTACCTGGCATTGCACCTCTCCCCATGCTGTCCGACTTCCTCCCGTCATTCCCTT
  TGGTTCCAGAGGGCTCTTCTGCAGCCAGCGCAGGGGAGCCGCTGTCTTCCCTTCCTGCCATGGGCCCACC
  TTCTGACCCTGTCATGACTACTGCAAAGGCAGACGCCTCTTCCCTCACTGTGGATGTGACGTCTCCTGCT
  TCCAAGGTCCCCACCACTGTTGAGGACAGAGTCAGCGACTGCACCCCGGCCGTCGAGAAGCCTGTGTCTG
  ATGCAGATGCTTCAGAGCCTTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027352
• Insert Size: 1356 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027352.4, NP_081628.3
• RefSeq Size: 2287 bp
• RefSeq ORF: 1356 bp
• Locus ID: 70231
• UniProt ID: Q99JX3
• Cytogenetics: 2 C2
• Gene Summary: Plays a role in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed:28617811). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (By similarity). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (PubMed:28617811). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) is the protein-coding transcript.