Hnrnph1 (NM_021510) Mouse Untagged Clone

CAT#: MC215993

Hnrnph1 (untagged) - Mouse heterogeneous nuclear ribonucleoprotein H1 (Hnrnph1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hnrnph1
• Synonyms: AI642080; E430005G16Rik; Hnrnph; Hnrph1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215993 representing NM_021510
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGGGAGCAGAAGGCGGAGAGGGCTTCGTGGTGAAGGTCCGGGGCTTGCCCTGGTCCTGCTCCG
  CGGATGAAGTGCAGCGCTTTTTTTCTGACTGCAAAATTCAAAATGGGGCTCAAGGTATTCGTTTCATCTA
  CACCAGAGAAGGCAGACCAAGTGGCGAGGCTTTTGTTGAACTTGAATCAGAAGATGAAGTCAAATTGGCC
  TTGAAAAAAGACAGAGAAACTATGGGACACAGATATGTTGAAGTATTCAAGTCAAACAACGTTGAAATGG
  ATTGGGTGTTGAAGCATACTGGTCCAAATAGTCCTGACACAGCCAATGATGGCTTTGTACGGCTTAGAGG
  ACTCCCTTTTGGATGTAGCAAGGAAGAAATTGTTCAGTTCTTCTCAGGGTTGGAAATCGTGCCAAATGGG
  ATAACATTGCCGGTGGACTTCCAGGGGAGGAGTACGGGGGAGGCCTTCGTGCAGTTTGCTTCACAGGAAA
  TAGCTGAAAAGGCTCTAAAGAAACACAAGGAAAGAATAGGGCACAGGTATATTGAAATATTTAAGAGCAG
  TCGAGCTGAAGTTAGAACTCATTATGACCCACCACGAAAACTTATGGCCATGCAGCGGCCAGGTCCCTAT
  GACAGACCTGGGGCTGGCAGAGGGTATAACAGCATTGGCAGAGGAGCTGGTTTTGAGAGGATGAGGCGTG
  GTGCTTATGGTGGAGGCTATGGAGGCTATGATGATTATAATGGCTATAATGATGGCTATGGATTTGGTTC
  AGATAGATTTGGAAGAGACCTCAATTATTGTTTTTCAGGAATGTCTGACCACAGATATGGGGACGGTGGC
  TCTACTTTCCAGAGTACAACCGGACACTGTGTACACATGCGGGGATTACCATACAGAGCTACTGAGAATG
  ACATTTATAATTTTTTTTCACCACTCAACCCTGTGAGAGTACATATTGAGATTGGACCTGATGGCAGAGT
  AACTGGTGAAGCAGATGTTGAGTTCGCAACCCATGAAGATGCTGTTGCAGCTATGTCAAAAGATAAAGCA
  AATATGCAACACAGATATGTAGAGCTCTTCTTGAATTCTACAGCAGGAGCAAGCGGTGGTGCTTACGAAC
  ACAGATATGTAGAACTCTTCTTGAATTCTACAGCAGGAGCAAGCGGTGGTGCTTATGGTAGCCAAATGAT
  GGGAGGCATGGGCTTGTCAAACCAGTCCAGTTACGGTGGCCCAGCCAGCCAGCAGCTGAGTGGTGGTTAT
  GGAGGTGGTTACGGTGGCCAGAGCAGCATGAGCGGATATGACCAAGTTTTACAGGAAAACTCCAGTGATT
  TTCAATCAAACATTGCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021510
• Insert Size: 1350 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021510.2, NP_067485.1
• RefSeq Size: 2242 bp
• RefSeq ORF: 1350 bp
• Locus ID: 59013
• UniProt ID: O35737
• Cytogenetics: 11 B1.3
• Gene Summary: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, resulting in novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.