Syt7 (NM_173067) Mouse Untagged Clone

CAT#: MC215986

Syt7 (untagged) - Mouse synaptotagmin VII (Syt7), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syt7
• Synonyms: AI851541; B230112P13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215986 representing NM_173067
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGGGACCCGGAGGCGGCCAGCCCAGGGGCACCTACCCGCGATGTCCTGCTGGTCTCTGCAATCA
  TCACCGTCAGCCTTAGCGTCACTATCGTCCTCTGCGGCCTGTGCCACTGGTGTCAGCGCAAACTGGGCAA
  ACGCTACAAGAATTCCTTGGAGACGGTGGGCACGCCAGACTCGGGGCGTGGGCGCGGTGAGAAGAAAGCC
  ATCAACGACCTAGACAGAGACTTTTGGAATAACAATGAAAGCACAGTGCAGCAGAAATGGAGTTCCTATC
  CTCCCAAGGAGTTTATTCTAAACATTTCACCCTACGCCCCTTATGGCGACCCTCGACTGTCCCTCAAGTT
  GCCTGCAGGAGGGAAGGCTGTGAATACAGCCCCAGTGCCCGGCCAGACGCCACACGATGAGTCTGACCGC
  AGAACGGAGACCCGTTCCTCTGTCTCGGACCTCGTCAACTCCCTTACCAGCGAGATGCTCATGCTCTCCC
  CGGGTTCTGAGGAGGATGAGGCCCACGAGGGCTGCAGCCGAGAGAACCTGGGCCGAATCCAGTTCAGTGT
  TGGCTACAACTTCCAAGAGTCCACACTCACCGTGAAGGTCATGAAGGCCCAAGAGCTGCCAGCCAAGGAC
  TTCAGCGGTACTAGTGACCCCTTTGTCAAGATCTACCTGCTACCCGACAAGAAGCACAAACTGGAGACCA
  AGGTGAAGCGGAAGAATCTAAACCCGCACTGGAATGAGACCTTTCTATTTGAAGGGTTTCCCTACGAGAA
  AGTGGTGCAGAGGGTCCTCTACCTCCAGGTCCTGGATTATGACCGTTTCAGCCGCAATGACCCCATTGGG
  GAGGTGTCCATCCCTCTGAACAAGGTGGACCTGACCCAGATGCAGACCTTCTGGAAGGATCTGAAGCCAT
  GCAGCGATGGGAGTGGGAGCCGAGGGGAGCTGCTCTTGTCCCTCTGCTACAACCCCTCTGCCAACTCCAT
  CATCGTGAACATCATCAAAGCTCGAAACCTCAAAGCCATGGACATCGGGGGCACATCAGACCCCTATGTG
  AAGGTGTGGCTGATGTATAAAGACAAGCGGGTAGAGAAAAAGAAGACCGTGACAAAGAAGAGGAACCTGA
  ACCCCATCTTCAATGAGTCTTTCGCCTTCGACATACCCACGGAGAAGCTGAGGGAGACCACGATCATCAT
  CACTGTCATGGACAAAGACAAGCTCAGCCGCAACGACGTCATCGGCAAGATCTACCTGTCCTGGAAGAGC
  GGACCAGGTGAAGTGAAACACTGGAAGGACATGATCGCTCGTCCCCGGCAGCCTGTGGCCCAGTGGCACC
  AGCTGAAAGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173067
• Insert Size: 1344 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173067.3, NP_775090.1
• RefSeq Size: 6474 bp
• RefSeq ORF: 1344 bp
• Locus ID: 54525
• UniProt ID: Q9R0N7
• Cytogenetics: 19 A
• Gene Summary: Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory and synaptic vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. SYT7 binds Ca(2+) with high affinity and slow kinetics compared to other synaptotagmins (PubMed:26738595). Involved in Ca(2+)-triggered lysosomal exocytosis, a major component of the plasma membrane repair (By similarity). Ca(2+)-regulated delivery of lysosomal membranes to the cell surface is also involved in the phagocytic uptake of particles by macrophages (PubMed:16982801, PubMed:21041449). Ca(2+)-triggered lysosomal exocytosis also plays a role in bone remodeling by regulating secretory pathways in osteoclasts and osteoblasts (PubMed:18539119). Involved in cholesterol transport from lysosome to peroxisome by promoting membrane contacts between lysosomes and peroxisomes: probably acts by promoting vesicle fusion by binding phosphatidylinositol-4,5-bisphosphate on peroxisomal membranes (PubMed:25860611). Acts as a key mediator of synaptic facilitation, a process also named short-term synaptic potentiation: synaptic facilitation takes place at synapses with a low initial release probability and is caused by influx of Ca(2+) into the axon terminal after spike generation, increasing the release probability of neurotransmitters (PubMed:24569478, PubMed:26738595). Probably mediates synaptic facilitation by directly increasing the probability of release (PubMed:26738595). May also contribute to synaptic facilitation by regulating synaptic vesicle replenishment, a process required to ensure that synaptic vesicles are ready for the arrival of the next action potential: SYT7 is required for synaptic vesicle replenishment by acting as a sensor for Ca(2+) and by forming a complex with calmodulin (PubMed:24569478). Also acts as a regulator of Ca(2+)-dependent insulin and glucagon secretion in beta-cells (PubMed:18308938, PubMed:19171650). Triggers exocytosis by promoting fusion pore opening and fusion pore expansion in chromaffin cells (PubMed:20956309). Also regulates the secretion of some non-synaptic secretory granules of specialized cells (By similarity).[UniProtKB/Swiss-Prot Function]