Nr1h3 (NM_001177730) Mouse Untagged Clone

CAT#: MC215976

Nr1h3 (untagged) - Mouse nuclear receptor subfamily 1, group H, member 3 (Nr1h3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nr1h3
• Synonyms: AU018371; LXR; RLD1; Unr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215976 representing NM_001177730
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTGTGGCTGGAGGCCTCAATGCCTGATGTTTCTCCTGATTCTGCAACGGAGTTGTGGAAGACAG
  AACCTCAAGATGCAGGAGACCAGGGAGGCAACACTTGCATCCTCAGGGAGGAAGCCAGGATGCCCCAGTC
  AACTGGGGTTGCTTTAGGGATAGGGTTGGAGTCAGCAGAGCCTACAGCCCTGCTCCCCAGGGCAGAGACC
  CTCCCAGAGCCGACAGAGCTTCGTCCACAAAAGCGGAAAAAGGGCCCAGCCCCCAAAATGCTGGGGAACG
  AGCTGTGCAGTGTCTGTGGGGACAAAGCCTCTGGCTTCCATTACAACGTGCTGAGCTGCGAGGGCTGCAA
  GGGATTCTTCCGCCGCAGTGTCATCAAGGGAGCACGCTATGTCTGCCACAGCGGTGGCCACTGCCCCATG
  GACACCTACATGCGGCGGAAATGCCAGGAGTGTCGACTTCGCAAATGCCGCCAGGCAGGCATGAGGGAGG
  AGTGTGTGCTGTCAGAAGAACAGATCCGCTTGAAGAAACTGAAGCGGCAAGAAGAGGAACAGGCTCAAGC
  CACTTCGGTGTCCCCAAGGGTGTCCTCACCTCCTCAAGTCCTGCCACAGCTCAGCCCAGAGCAGCTGGGC
  ATGATCGAGAAGCTGGTGGCTGCCCAGCAACAGTGTAACAGGCGCTCCTTCTCAGACCGCCTGCGCGTCA
  CGCCTTGGCCCATTGCACCCGACCCTCAGAGCCGGGAAGCCCGACAACAGCGCTTTGCCCACTTTACTGA
  GCTGGCCATCGTGTCCGTGCAGGAGATTGTTGACTTTGCCAAACAGCTCCCTGGCTTCCTACAGCTCAGC
  AGGGAGGACCAGATCGCCTTGCTGAAGACCTCTGCAATCGAGGTCATGCTTCTGGAGACGTCACGGAGGT
  ACAACCCCGGCAGTGAGAGCATCACCTTCCTCAAGGACTTCAGTTACAACCGGGAAGACTTTGCCAAAGC
  AGGGCTGCAGGTGGAGTTCATCAACCCCATCTTTGAGTTCTCCAGAGCCATGAATGAGCTGCAACTCAAT
  GATGCTGAGTTTGCTCTGCTCATTGCCATCAGCATCTTCTCTGCAGACCGGCCCAACGTGCAGGACCAGC
  TCCAAGTAGAGAGGCTGCAACACACATATGTGGAGGCCCTGCACGCCTACGTCTCCATCAACCACCCCCA
  CGACCCACTGATGTTCCCACGGATGCTAATGAAGCTGGTGAGCCTCCGTACTTTGAGCAGCGTCCATTCA
  GAGCAAGTGTTTGCCCTTCGCCTGCAGGACAAAAAGCTTCCCCCTCTGCTGTCTGAGATCTGGGATGTCC
  ACGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177730
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177730.1, NP_001171201.1
• RefSeq Size: 1802 bp
• RefSeq ORF: 1338 bp
• Locus ID: 22259
• UniProt ID: Q9Z0Y9
• Cytogenetics: 2 50.52 cM
• Gene Summary: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:18055760, PubMed:19520913, PubMed:20427281). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a different segment for its 5' UTR, compared to variant 1. Variants 1, 2, and 3 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.