Tead2 (NM_011565) Mouse Untagged Clone

CAT#: MC215970

Tead2 (untagged) - Mouse TEA domain family member 2 (Tead2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tead2
• Synonyms: Etdf; ETF; TEAD-2; TEF-4; TEF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215970 representing NM_011565
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATCCCCGGACTGGGGCCCCCCTGGATGATGGCGGGGGCTGGACAGGTAGCGAGGAAGGCAGCG
  AAGAGGGCACCGGAGGCAGCGAGGGCGTTGGAGGTGATGGCAGCCCTGATGCAGAAGGTGTGTGGAGCCC
  CGACATTGAGCAGAGTTTTCAGGAGGCCCTGGCCATCTACCCTCCCTGTGGCCGTCGCAAGATCATCCTG
  TCAGATGAGGGCAAGATGTATGGCCGGAACGAACTCATTGCCCGTTACATCAAGCTGAGGACAGGGAAGA
  CGAGAACGCGAAAGCAGGTCTCCAGCCATATTCAGGTTTTGGCTCGAAGGAAATCGAGAGAAATTCAGTC
  CAAGCTGAAGGACCAAGTCTCCAAGGACAAGGCCTTCCAGACGATGGCCACCATGTCTTCGGCACAGCTC
  ATCTCCGCCCCTTCCCTCCAGGCCAAGCTGGGCCCTTCTGGCCCTCAGGCCACTGAGCTTTTCCAGTTCT
  GGTCAGGGAGCTCTGGGCCACCATGGAATGTTCCAGACGTGAAGCCCTTCTCACAGGCACCGTTCTCCGT
  GTCACTGACGCCCCCAGCCTCTGACCTACCAGGGTACGAGCCGCCCCCAGCCCTCTCACCCCTGCCCCCA
  CCCGCTCCGTCTCCCCCAGCCTGGCAGGCTCGGGCCCTGGGCACTGCCCGCCTGCAGCTGATAGAGTTCT
  CAGCGTTTGTGGAACCGCCAGACGCAGTTGACTCGTTCCAGAGGCATCTGTTTGTCCACATCAGTCAGCA
  GTGTCCCAGCCCTGGAGCACCACCCCTAGAGAGTGTGGACGTGCGGCAGATCTACGACAAATTCCCTGAG
  AAGAAGGGCGGCCTCCGCGAGCTGTATGACCGAGGGCCACCACATGCCTTCTTCCTCGTCAAGTTCTGGG
  CGGACCTGAACTGGGGCCCCAGTGCCGAGGAGGCAGGGAGCAGCGGAGGTGGCGGTGGCTTCTATGGAGT
  GAGCAGCCAGTATGAGAGCCGGGAGCTCATGACACTCACCTGCTCCTCCAAGGTCTGCTCCTTTGGCAAG
  CAAGTGGTAGAGAAGGTGGAGACGGAACGGGCCCAGCTGGAGGACGGGCGCTTTGTGTACCGTCTGCTGC
  GCTCTCCCATGTGTGAGTACCTGGTTAATTTCCTGCACAAGCTCCGTCAGCTGCCTGAACGCTACATGAT
  GAACAGTGTCCTGGAGAACTTCACCATCCTCCAGGTTGTGACAAACAGGGACACTCAGGAACTGCTGCTG
  TGTACTGCCTACGTCTTTGAAGTCTCCACCAGTGAACGAGGAGCCCAGTACCACATCTACCGCCTGGTCA
  GGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011565
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011565.2, NP_035695.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1338 bp
• Locus ID: 21677
• UniProt ID: P48301
• Cytogenetics: 7 29.19 cM
• Gene Summary: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction (By similarity). Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).