Nfatc2 (NM_001037178) Mouse Untagged Clone

CAT#: MC215954

Nfatc2 (untagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (Nfatc2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfatc2
• Synonyms: AI607462; NF-ATc2; NF-ATp; NFAT1; NFAT1-D; Nfatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215954 representing NM_001037178
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTCGAACCAGCCTCGCCGAGGACAGCTGCCTGGGCCGACACTCGCCCGTGCCCCGTCCGGCAT
  CCCGCTCCTCCTCACCCGGTGCCAAGCGGAGGCATTCGTGCGCAGAGGCTTTGGTTGCTCCTCTGCCCGC
  AGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCCACAGCCCTCGCCTCACGTGGCACTGCAGGACGACAGC
  ATCCCCGCTGGGTACCCCCCCACGGCCGGCTCTGCTGTTCTCATGGATGCCCTCAACACCCTGGCCACCG
  ACTCGCCCTGCGGGATCCCCTCCAAGATATGGAAGACCAGTCCTGACCCGACGCCTGTGTCCACCGCTCC
  GTCCAAGGCTGGCCTGGCCCGCCACATCTACCCTACTGTGGAGTTCCTGGGGCCATGTGAGCAGGAGGAG
  AGGAGGAATTCCGCTCCAGAGTCCATCCTGCTGGTACCACCTACTTGGCCCAAGCAGTTGGTGCCGGCCA
  TTCCCATCTGCAGCATCCCTGTGACTGCATCCCTCCCACCACTCGAGTGGCCACTCTCCAATCAGTCGGG
  CTCCTATGAGCTACGGATTGAGGTCCAACCCAAGCCCCATCACCGGGCCCACTATGAGACGGAGGGCAGC
  CGTGGCGCTGTCAAAGCCCCAACAGGAGGACACCCTGTGGTGCAGCTCCACGGCTACATGGAGAACAAGC
  CTCTGGGGCTTCAGATCTTCATTGGGACAGCAGATGAGAGGATCCTTAAGCCGCACGCCTTCTACCAAGT
  ACACAGGATCACTGGGAAAACGGTCACCACCACGAGCTATGAGAAGATCGTAGGCAACACCAAGGTCCTG
  GAGATCCCCCTGGAGCCAAAGAACAACATGAGAGCCACCATCGACTGTGCAGGCATCCTGAAGCTCCGAA
  ACGCTGACATCGAGCTGCGGAAGGGCGAGACGGACATCGGCAGGAAGAACACGCGTGTGCGCCTGGTGTT
  CCGCGTGCACGTCCCAGAGCCCAGTGGGCGCATCGTCTCCCTGCAGGCTGCGTCCAACCCCATCGAGTGC
  TCTCAGCGCTCTGCCCACGAGCTGCCCATGGTGGAGAGACAAGACATGGACAGCTGCCTGGTCTACGGGG
  GCCAGCAGATGATCCTCACGGGCCAGAACTTCACAGCGGAGTCCAAGGTTGTGTTCATGGAGAAGACTAC
  AGGACCTGCAGGGACCTGTGAGACTCGGCCTTTGCCCATCTCTCTGATCTCAGCTGACCGTCTCTCCCCC
  TGGCTTTCCCGGCTGCAGAGAAACCCTCCTGGCTCTGTCTTCAGATGCTCCGTGCTCCTTCCTGCCCCAG
  GATCTTCGCTTGTGCTGCTGGCTCTGTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001037178
• Insert Size: 1359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037178.2, NP_001032255.1
• RefSeq Size: 2763 bp
• RefSeq ORF: 1359 bp
• Locus ID: 18019
• Cytogenetics: 2 88.91 cM
• Gene Summary: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3, also known as IA-IIS-VIIa) contains alternate 5' and 3' exon structure, and it thus differs in both UTRs, initiates translation at a downstream in-frame start codon, and differs in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform a. Both variants 3 and 16 encode isoform c.