F7 (NM_010172) Mouse Untagged Clone

CAT#: MC215931

F7 (untagged) - Mouse coagulation factor VII (F7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: F7
• Synonyms: AI132620; Cf7; F; FVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215931 representing NM_010172
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCCACAGGCGCATGGGCTGCTGCTTCTCTGCTTTCTGCTCCAGCTCCAGGGACCTCTAGGGACTG
  CAGTTTTCATAACCCAGGAGGAAGCACATGGTGTCCTACACAGGCAAAGGCGTGCCAACTCACTCCTGGA
  GGAGCTTTGGCCCGGCTCTCTGGAGAGAGAGTGCAATGAGGAACAGTGCTCCTTTGAGGAGGCCCGGGAG
  ATCTTCAAGAGCCCTGAGAGGACCAAGCAGTTCTGGATTGTTTACAGTGATGGGGACCAGTGTGCCTCGA
  ATCCATGTCAGAACGGAGGTACCTGCCAGGATCATCTCAAGTCTTACGTCTGCTTCTGCCTCCTAGACTT
  TGAGGGTCGGAACTGTGAGAAAAGCAAGAATGAGCAGCTGATCTGTGCAAATGAAAATGGTGACTGTGAC
  CAGTACTGCAGGGACCATGTAGGGACCAAGCGTACCTGTAGCTGTCATGAGGACTACACGCTACAGCCAG
  ATGAGGTGTCCTGCAAACCAAAAGTTGAGTACCCGTGTGGGAGAATACCTGTTGTAGAAAAAAGAAACTC
  CAGCAGCCGCCAAGGCCGCATTGTGGGAGGCAACGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGCTGTG
  CTGAAAATCAATGGGTTATTGCTGTGTGGGGCCGTCCTGCTGGACGCCAGATGGATAGTGACCGCAGCCC
  ACTGCTTCGATAATATCCGCTACTGGGGAAACATCACAGTGGTGATGGGTGAACATGACTTCAGTGAGAA
  GGATGGGGATGAGCAAGTACGACGGGTGACACAGGTCATCATGCCCGACAAGTACATCCGCGGCAAGATC
  AACCACGACATTGCCCTGCTCCGCCTTCACCGGCCTGTGACCTTCACTGACTACGTGGTGCCCCTGTGTC
  TGCCTGAAAAGTCCTTCTCCGAGAACACCCTAGCCAGAATCCGCTTCTCAAGGGTCAGTGGCTGGGGCCA
  GCTACTGGACCGTGGTGCCACAGCCCTGGAACTCATGTCCATCGAGGTGCCCCGGCTGATGACCCAGGAC
  TGTCTGGAGCACGCCAAGCACAGCTCTAACACCCCCAAAATCACAGAGAACATGTTCTGCGCTGGCTACA
  TGGATGGTACCAAGGACGCCTGCAAGGGTGACAGCGGTGGCCCACATGCCACGCACTACCATGGCACATG
  GTATCTGACAGGTGTGGTCAGCTGGGGGGAGGGCTGTGCAGCTATTGGTCACATTGGGGTGTACACCAGG
  GTCTCCCAGTACATAGACTGGCTGGTCAGACACATGGACTCCAAGCTCCAGGTTGGGGTTTTCCGACTCC
  CACTACTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010172
• Insert Size: 1341 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC061149, AAH61149
• RefSeq Size: 1869 bp
• RefSeq ORF: 1341 bp
• Locus ID: 14068
• UniProt ID: P70375
• Cytogenetics: 8 5.73 cM
• Gene Summary: This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels. [provided by RefSeq, Apr 2015]