Dapk3 (NM_001190473) Mouse Untagged Clone

CAT#: MC215924

Dapk3 (untagged) - Mouse death-associated protein kinase 3 (Dapk3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dapk3
• Synonyms: dlk; ZIPK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215924 representing NM_001190473
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACATTCAGGCAAGAGGATGTTGAGGACCATTATGAGATGGGAGAGGAGCTTGGCAGTGGCCAAT
  TTGCCATCGTGCGCAAGTGCCAGCAGAAGGGCACGGGCATGGAGTATGCAGCCAAGTTCATCAAGAAGCG
  GCGCCTGCCATCCAGCCGGCGCGGTGTGAGCCGGGAGGAGATCGAACGCGAGGTGAGCATCCTGCGCGAG
  ATCCGCCACCCCAACATCATAACACTGCATGACGTGTTCGAGAACAAGACAGATGTGGTGCTGATCCTGG
  AGCTGGTGTCCGGTGGCGAGCTTTTCGACTTCCTGGCCGAGAAGGAGTCATTGACGGAGGATGAGGCCAC
  GCAGTTCCTCAAACAAATCCTAGACGGTGTCCACTACCTGCACTCCAAGCGCATCGCACACTTTGACCTG
  AAGCCCGAGAACATCATGTTGCTGGACAAGCACGCAGCCAGCCCCCGCATTAAGCTCATCGACTTTGGCA
  TCGCGCACAGGATCGAGGCTGGCAGCGAGTTCAAGAACATCTTTGGCACACCCGAGTTTGTCGCCCCCGA
  GATCGTGAACTATGAGCCACTTGGCTTGGAGGCTGACATGTGGAGCATTGGCGTCATCACCTACATCCTC
  CTGAGCGGAGCGTCCCCATTCCTGGGCGAGACCAAGCAGGAGACGCTGACGAACATCTCAGCAGTGAACT
  ATGACTTTGATGAGGAATACTTCAGCAGCACCAGCGAGCTGGCCAAGGACTTCATCCGCAGGCTGCTGGT
  CAAAGACCCCAAGAGGAGGATGACCATCGCACAGAGCCTGGAGCATTCCTGGATCAAGGTGCGCAGGCGC
  GAGGACGGCGCCCGGAAGCCAGAGCGACGGCGGCTGCGCGCCGCGCGCCTGCGCGAGTACAGCCTCAAGT
  CCCACTCGAGCATGCCGCGCAACACGAGCTACGCCAGCTTCGAGCGCTTCTCACGCGTGCTGGAGGACGT
  GGCGGCGGCAGAGCAGGGGCTGCGCGAGCTGCAGCGAGGCAGGCGCCAGTGCCGGGAGCGCGTGTGTGCG
  CTGCGCGCGGCCGCCGAGCAGCGGGAGGCGCGCTGCCGCGACGGGAGCGCAGGGCTAGGGCGCGACCTGC
  GACGCCTGCGCACGGAGCTGGGGCGCACCGAGGCTCTGCGCACGCGCGCGCAGGAGGAGGCGCGGGCGGC
  GCTGTTGGGTGCCGGGGGCCTGAAGCGTCGCCTGTGTCGCCTGGAGAACCGTTACGACGCGCTAGCCGCT
  CAGGTGGCCGCTGAGGTGCAATTCGTGCGCGACCTGGTGCGTGCGCTGGAGCAGGAACGGCTGCAGGCTG
  AGTGCGGCGTGCGCTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001190473
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190473.1, NP_001177402.1
• RefSeq Size: 1676 bp
• RefSeq ORF: 1347 bp
• Locus ID: 13144
• UniProt ID: O54784
• Cytogenetics: 10 39.72 cM
• Gene Summary: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation; in vitro phosphorylates PAWR (By similarity). Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2; disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis (By similarity). Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.