Bcl3 (NM_033601) Mouse Untagged Clone

CAT#: MC215920

Bcl3 (untagged) - Mouse B-cell leukemia/lymphoma 3 (Bcl3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bcl3
• Synonyms: AI528691; Bcl-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215920 representing NM_033601
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGATGCCCCGCGGGGGCCATGGACGAGGGGCCCGTGGACCTGCGCACCCGGCCCAAGGGCACCC
  CGGGCGCCGCGCTGCCACTCCGCAAACGCCCGCTGCGCCCCGCGTCCCCAGAGCCCGCAACCACGCGCAG
  CCCCGCTGGACCCCTGGACGCCCTGCGCAGCGGCTGCGACGTCCCGGTTGTCCCTGGGCCCCCCCACTGT
  GTGGCCAGGCCGGAGGCCCTTTACTACCAGGGACCTTTGATGCCCATTTACTCTACCCCGACGATGGCCC
  CCCACTTTCCGCTGCTGAACCTGCCTACTCACCCCTACTCCATGATATGCCCCATGGAACACCCCCTTTC
  AGCTGATATTGCCATGGCCACTCGGGTGGATGAGGATGGAGACACGCCTCTCCACATCGCTGTGGTCCAG
  AATAACATAGCCGCTGTCTACCGAATACTCAGCCTTTTCAAGCTTGGGAGCCGCGAAGTAGACGTCCATA
  ACAACCTGCGGCAGACCCCGCTCCACCTGGCTGTCATCACCACATTACCAGACATGGTCCGGCTCTTGGT
  GACAGCTGGTGCCAGCCCCATGGCCCTGGATCGTCACGGCCAGACTGCAATTCACCTGGCATGCGAGCAC
  CGCAGCCCCAGCTGCCTGCAGGCCCTGCTGGACAGCGCAACCTCAGGCTCCGTGGACCTGGAGGTTCGCA
  ATTATGAAGGGCTCACTGCCCTGCACGTGGCCGTGAACACCGGGTGCCAGGAGGCTGTTCTGCTGCTCCT
  GGAGCGTGGCGCGGACATCGATGCAGTGGATATCAAGAGCGGCCGCTCCCCACTCATCCACGCCGTGGAG
  AACAACAGCCTGAACATGGTGCAACTCCTGCTGCTGCACGGCGCCAACGTGAACGCTCAGATGTATTCTG
  GCAGCTCGGCTCTGCATTCTGCGTCTGGCCGCGGGCTTCTGCCTCTGGTGCGCACGCTGGTGCGCAGCGG
  GGCTGACAGCGGCCTCAAGAACTGTCACAATGACACACCTCTCATGGTGGCGCGCAGCCGCAGGGTCATT
  GATATCTTAAGGGGGAAGGCCTCTCGGGCTGCTTCAGGGTCACAGCCTGAGCCATCCCCAGACCAAAGTG
  CCACCAACTCCCCCGAGAGCAGCAGTCGTCTCAGCTCCAATGGCCTCCAGTCCTCGCCAAGCTCCTCACC
  CTCGCTGTCTCCCCCGAAAGATGCTCCTGGCTTCCCCGCGACTCCCCAAAACTTTTTCCTTCCCACAACA
  TCTACACCTGCCTTCCTGCCCTTCCCAGGAGTCCTCCGAGGCCCTGGCCGGCCCGTACCCCCTTCCCCAG
  CTCCAGGAAGCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033601
• Insert Size: 1347 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033601.3, NP_291079.2
• RefSeq Size: 1835 bp
• RefSeq ORF: 1347 bp
• Locus ID: 12051
• UniProt ID: Q9Z2F6
• Cytogenetics: 7 9.95 cM
• Gene Summary: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit (By similarity). In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.[UniProtKB/Swiss-Prot Function]