Peli3 (NM_172835) Mouse Untagged Clone

CAT#: MC215911

Peli3 (untagged) - Mouse pellino 3 (Peli3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Peli3
• Synonyms: 6030441F14Rik; A930011L17; BC028931
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215911 representing NM_172835
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGGAAGGAAACCCTGACGTGGGATCCCCCCGGACCTCAGACCTCCAGCACCCGGGGAGCCAGG
  GCTCTTGCATCCTGTCTTGTCCTGGTGAAGAAGCACTGGCAGGCGAGGAGCCCATCAAGTATGGTGAACT
  CATCGTTCTGGGCTACAATGGGTGTCTGGCAAGTGGAGACAAGGGCCGCCGCCGAAGCCGCCTGGCACTG
  AGCCGCCGGCCACATGCCAACGGAGTGAAGCCAGATGTCATGCACCACATCTCCACACCACTCGTCTCCA
  AGGCCCTGAGTAACCGAGGCCAGCACAGCATCTCATTCACACTGTCCCGGAGTCACTCAGTCATAGTGGA
  GTACACACATGACAGCGACAAAGACATGTTCCAGATTGGCCGCTCTACTGAAAACATGATTGACTTTGTG
  GTAACAGACACATCTCCTGGTGGAGGGGCTACAGAGGGCCCTTCTGCCCAAAGTACCATCTCCCGCTATG
  CCTGCAGAATTCTCTGTGACCGCCGGCCACCCTATACTGCCCGAATCTACGCTGCTGGCTTTGATGCCTC
  TAGCAACATCTTTCTTGGAGAGCGGGCAGCCAAATGGAGGACTCCGGATGGTCTGATGGACGGCTTGACA
  ACCAATGGGGTTCTGGTGATGCACCCAGCAGGTGGCTTCTCCGAGGACTCTGCCCCAGGTGTCTGGAGGG
  AGATTTCTGTCTGCGGGAATGTGTACACGCTGCGGGATAGCCGATCAGCTCAGCAGCGGGGGAAGCTGGT
  GGAAAACGAATCCAACGTCCTGCAAGACGGTTCGCTCATCGACCTGTGTGGGGCCACACTGTTGTGGCGC
  ACTCCGGCTGGGTTGCTGAGGGCACCCACACTGAAACAACTAGAGGCCCAGCGACAGGAGGCCAACGCAG
  CACGGCCCCAGTGCCCCGTGGGCCTTAGCACCTTGGCCTTCCCCAGCCCAGCCCGTGGCCGCACAGCACC
  CGACAAACAGCAACCCTGGGTCTACGTCCGTTGTGGTCATGTCCATGGCTATCACGGCTGGGGCTGCCGG
  AGGGAACAAGGCCCCCAGGAGCGAGAGTGTCCTCTCTGCCGCCTTGTGGGACCCTATGTGCCCCTGTGGC
  TCGGTCAGGAGGCCGGTCTCTGCCTGGACCCTGGGCCACCCAGCCACGCTTTTGCACCCTGTGGCCACGT
  CTGTTCTGAGAAGACTGCCCGCTACTGGGCTCAGACACCGCTGCCGCACGGCACCCATGCTTTCCACGCT
  GCCTGCCCCTTCTGTGGAGCTTGGCTCACCGGTGAGCTTGGCTGTGTCCGCCTCATTTTCCAGGGGCCAC
  TGGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172835
• Insert Size: 1338 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172835.3, NP_766423.3
• RefSeq Size: 1937 bp
• RefSeq ORF: 1338 bp
• Locus ID: 240518
• UniProt ID: Q8BXR6
• Cytogenetics: 19 A
• Gene Summary: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation.[UniProtKB/Swiss-Prot Function]