Sde2 (NM_145943) Mouse Untagged Clone

CAT#: MC215908

Sde2 (untagged) - Mouse cDNA sequence BC031781 (BC031781), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sde2
• Synonyms: MGC30618
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215908 representing NM_145943
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCTGCGGTGGTGGCGTGGGTTCGCGGCCCCGGTACCGTGTGGAAGGCGCTGCCGTGTGCCT
  CGGTTGGGTGCTCCGTGCGCGATGTGATCTACCGACACTGCCAGGAACAGGAAGTTCCCGTGGAATGCTT
  CTTTGTGACGTGCAATGGAGTCCTCGTTAATGCCGGTGATAAGGTGCAGCATGGAGCCGTGTACAGTTTG
  GAGCCCAGGCTTCGTGGGGGGAAAGGAGGTTTTGGGTCTATGCTTCGAGCACTTGGTGCACAGATAGAGA
  AGACAACCAATAGAGAAGCCTGCCGGGATCTCAGTGGGAGGAGATTACGAGATGTCAATCATGAGAAAGC
  GATGGCTGAGTGGGTAAAACAGCAAGCTGAGCGAGAGGCTGAAAAGGAGCAAAGGCGCCTGGAGAGGCTG
  CAGCGAAAGCTTGCCGAGCCTGCACACTGCTTCACCAGTCCCGACTACCAGAGGCAGTGCCATGAGATGG
  CTGAGCGTCTGGAGGACTCAGTCCTCAAAGGTATGCAGGCTGCATCCAGCAAGATGGTATCAGCAGAAAT
  CACTGAGACTCGGAAACGGCCTAACAAGTCAAAAACGGACCAGGAAACTAGTGCAAAAAAAAGGAAAAGG
  AAATGCTTTTGGTTGGGCATGGATGGGCTGGAGGCTGCCGAGGGGTCCAGTACTGGAAGCTCAGAGGACA
  GCTCAGAGGATGACAGTGAGGATGCTCCTGGGACTTCGGAGCAGAGCTGCTGTGCTCGGGAAGACGGCAT
  TGATGCTGTAGAGGTGGCAGCCGACCGACCTGGCAGTCCTCGGAGCTCGGCCTCTGGTACACACTCTGAG
  TCACCTGAAAAGCTTCAGTGCCCCGTGACCGAGCCTGGGCAGGGCATTTTGGAGAACACAGGAACTGAGC
  CAGGAGAGACCTCTGACAAGGAGTGCAACGAGAGGAAGACAGTCACAGACCCAGAGGAGACGCCAGCAAG
  GAAGGAAACAGAGAGTCATGAGGCCACAGAAAAGGACCAAAAGACAGGAATGTCTGGTGGGGACAGAGCT
  GCCATGGTTTTGTCTGGAGAAGACAGGAAAAGTGTCCCTGCTGCTAATCTGGAGGGAAACAACTCAGGAG
  ACACAGCTCTTGGTCTGGAAGCGGTGGATCTGTCAGCATTCAGCTCTGCAGCCGAGTTGGAGTCGCTGGG
  TTTGGAGAGGCTCAAGTGTGAACTGATGGTCCTTGGACTGAAGTGTGGGGGGACCCTGCAGGAGCGCGCA
  GCCAGGCTCTTCTCTGTCAGAGGCCTCACAAAGGAACTCATTGACCCAGCTTTATTTGCCAAGCCTTCCA
  AGGGGAAGAAGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145943
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145943.1, NP_666055.1
• RefSeq Size: 3203 bp
• RefSeq ORF: 1347 bp
• Locus ID: 208768
• UniProt ID: Q8K1J5
• Cytogenetics: 1 H4
• Gene Summary: Involved in both DNA replication and cell cycle control. Unprocessed SDE2 interacts with PCNA via its PIP-box. The interaction with PCNA prevents monoubiquitination of the latter thereby inhibiting translesion DNA synthesis. The binding of SDE2 to PCNA also leads to processing of SDE2 by an unidentified deubiquitinating enzyme, cleaving off the N-terminal ubiquitin-like domain. The resulting mature SDE2 is degraded by the DCX(DTL) complex in a cell cycle- and DNA damage dependent manner. Binding of SDE2 to PCNA is necessary to counteract damage due to ultraviolet light induced replication stress. The complete degradation of SDE2 is necessary to allow S-phase progression.[UniProtKB/Swiss-Prot Function]