Gnal (NM_177137) Mouse Untagged Clone

CAT#: MC215899

Gnal (untagged) - Mouse guanine nucleotide binding protein, alpha stimulating, olfactory type (Gnal), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gnal
• Synonyms: 2610011C15Rik; 9630020G10Rik; Galphaolf; Gna10; Golf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215899 representing NM_177137
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTATGCTACAGCCTGCGGCCGCTGCTCTTCGGGAGCCCAGAGGACACCCCGTGTGCGGCCTCGG
  AACCCTGCGCAGAGGATGCTCAGCCCAGCGCCGCCCCGGCCCCTGCCTCGATCCCAGCCCCGGCTCCCGT
  AGGGACCCTGCTCCGGCGTGGCGGCGGCCGGATCGTCGCGAACGCGCGGCCGCCAGGCGAGCTGCAGAGC
  CGCCGGCGACAGGAGCAGCTACGAGCCGAGGAGCGCGAGGCGGCTAAAGAGGCGAGGAAAGTCAGCCGGG
  GCATCGACCGCATGCTGCGCGAGCAGAAGCGGGACCTGCAGCAGACGCACCGGCTCCTGCTGCTGGGGGC
  TGGTGAGTCCGGGAAAAGCACTATCGTCAAACAGATGAGGATCCTGCACGTCAATGGCTTCAACCCCGAG
  GAAAAGAAGCAGAAAATTCTGGACATCAGGAAAAATGTCAAAGATGCGATCGTGACAATCGTTTCAGCAA
  TGAGTACTATCATACCTCCAGTTCCACTGGCCAACCCTGAGAACCAGTTCCGGTCAGATTATATCAAGAG
  CATAGCCCCTATCACTGACTTTGAATATTCCCAGGAGTTCTTTGACCATGTGAAGAAGCTGTGGGACGAT
  GAAGGAGTGAAGGCCTGCTTTGAGAGATCCAACGAGTACCAGCTGATCGACTGTGCACAATACTTCCTGG
  AAAGGATTGACAGTGTCAGTCTGGTTGACTACACACCCACAGACCAGGACCTGCTCAGATGCAGAGTGCT
  GACATCAGGAATCTTTGAGACACGATTCCAAGTGGACAAAGTGAACTTTCACATGTTTGATGTTGGAGGC
  CAGAGAGATGAGAGAAGAAAATGGATCCAGTGTTTTAATGATGTCACTGCGATCATTTACGTGGCGGCCT
  GTAGTAGCTACAACATGGTGATCCGGGAAGATAACAATACCAACAGACTTCGGGAATCACTGGACCTGTT
  TGAAAGCATCTGGAATAACAGGTGGTTGCGAACCATTTCTATCATCCTATTCTTGAACAAACAAGACATG
  CTGGCAGAAAAAGTCTTGGCAGGGAAGTCAAAAATCGAAGACTATTTCCCGGAGTATGCCAATTATACTG
  TCCCTGAAGATGCAACACCAGATGCGGGAGAAGATCCCAAAGTTACAAGAGCAAAGTTCTTTATCCGGGA
  TCTGTTCTTGAGGATCAGCACAGCCACGGGTGATGGCAAACATTACTGCTACCCTCACTTCACCTGCGCC
  GTGGACACAGAGAACATCCGCAGAGTGTTCAACGATTGCCGTGACATCATCCAGAGAATGCATCTCAAGC
  AGTACGAACTCTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177137
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177137.5, NP_796111.2
• RefSeq Size: 5654 bp
• RefSeq ORF: 1347 bp
• Locus ID: 14680
• Cytogenetics: 18 39.85 cM
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.