Enpp6 (NM_177304) Mouse Untagged Clone

CAT#: MC215887

Enpp6 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 6 (Enpp6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Enpp6
• Synonyms: 4833421B01Rik; B830047L21Rik; D8Ertd514; D8Ertd514e; E-NPP 6; GPC-Cpde; N; Npp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215887 representing NM_177304
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAAAGCTCTGGACCTTCCTGCTGGGCTTTGGGCTCAGCTGGGTGTGGCCGGCTTCTGCCCACC
  GGAAGCTCCTGGTGTTGCTCCTGGATGGTTTTCGCTCAGACTACATCAGTGAGGATGCTCTAGCATCCTT
  GCCTGGCTTCAGAGAGATTGTGAACAGAGGCGTCAAAGTGGATTACTTGACTCCAGACTTTCCCAGCCTC
  TCCTATCCCAATTACTACACCCTCATGACTGGCCGCCACTGTGAGGTCCACCAGATGATCGGCAACTACA
  TGTGGGATCCCAGAACCAACAAGTCATTTGACATCGGGGTCAACCGAGACAGCCTGATGCCCCTGTGGTG
  GAACGGGTCAGAACCGCTGTGGATCACTCTGATGAAAGCCAGGAGGAAAGTCTACATGTATTACTGGCCC
  GGCTGTGAAGTTGAGATTCTTGGTGTCAGACCAACTTACTGCCTAGAATATAAAACTGTCCCAACAGATA
  TCAACTTTGCGAATGCAGTTAGCGATGCTCTCGACTCATTAAAGAGTGGCCGAGCGGATCTAGCAGCCAT
  ATACCATGAGCGCATCGATGTAGAAGGTCACCACTACGGCCCTTCATCACCTCAGAGAAAAGATGCCCTC
  AGAGCTGTGGACACTGTCCTGAAGTATATGATCCAGTGGATTCAGGACCGAGGCCTGCAGCAGGACCTAA
  ACGTCATCCTCTTCTCAGACCATGGGATGACTGACATCTTCTGGATGGATAAAGTGATTGAGCTGAGCAA
  CTACATCAGCCTGGACGACCTGCAGCAAGTGAAAGACCGAGGGCCCGTTGTGAGCCTGTGGCCAGTTCCT
  GGAAAACACTCTGAGATATATCACAAACTCCGCACAGTGGAACACATGACCGTGTATGAGAAAGAATCAA
  TACCCAACAGGTTCTATTACAAGAAAGGAAAATTTGTCTCTCCTTTGACCCTGGTGGCTGATGAAGGATG
  GTTCATAGCAGAGAGTCGAGAGATGCTTCCATTTTGGATGAACAGCACGGGCAAGCGTGAAGGCTGGCAG
  CGAGGATGGCACGGATATGACAACGAACTCATGGACATGAGAGGCATCTTCCTGGCCATCGGACCTGATT
  TCAAGTCCAACTTCAGAGCTGCTCCAATCAGATCCGTGGATGTCTACAACATCATGTGCCACGTCGCAGG
  CATCACCCCACTGCCCAACAACGGGTCCTGGTCCAGGGTGGTATGCATGCTGAAGGGCCAGACCAGCTCT
  GCTCCACCCACCCCACTGAACAGCTGTGCACTGGTCTTGATTCTCCTCTTATACTTTGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177304
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177304.3, NP_796278.1
• RefSeq Size: 3065 bp
• RefSeq ORF: 1323 bp
• Locus ID: 320981
• UniProt ID: Q8BGN3
• Cytogenetics: 8 26.48 cM
• Gene Summary: This gene encodes a member of the nucleotide pyrophosphatase/phosphodiesterase family of enzymes that play an important role in regulating extracellular nucleotide metabolism. The encoded preproprotein undergoes proteolytic processing to generate a glycosylphosphatidylinositol (GPI)-anchored membrane protein that hydrolyzes choline-containing lysophospholipids such as glycerophosphocholine. Mice lacking the encoded protein develop fatty liver and myelin sheath abnormalities. [provided by RefSeq, Jul 2016]