Sept7 (NM_009859) Mouse Untagged Clone

CAT#: MC215879

41524 (untagged) - Mouse septin 7 (Sept7), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sept7
• Synonyms: Cdc10; E430034N22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215879 representing NM_009859
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACCATGGTAGCTCAAC
  CGAAGAACCTTGAGGGCTATGTGGGCTTTGCCAACCTCCCAAATCAAGTGTACAGAAAATCCGTGAAAAG
  AGGATTTGAATTCACTCTTATGGTAGTAGGTGAATCTGGACTGGGAAAGTCGACATTAATCAACTCATTA
  TTCCTCACAGATTTGTATTCTCCAGAGTATCCAGGACCTTCTCATAGAATCAAAAAGACTGTACAGGTGG
  AGCAATCCAAAGTTTTAATCAAAGAAGGTGGTGTTCAGTTGCTGCTGACAATAGTTGATACTCCAGGATT
  TGGAGATGCAGTGGATAATAGTAATTGCTGGCAGCCTGTTATCGACTACATTGATAGTAAATTTGAAGAT
  TACTTAAATGCAGAATCTCGAGTGAACAGACGTCAGATGCCTGATAACAGGGTGCAGTGTTGTTTATACT
  TCATTGCTCCTTCAGGACATGGACTTAAACCATTGGACATTGAGTTTATGAAACGTTTGCATGAAAAAGT
  GAATATCATCCCATTAATTGCCAAAGCAGACACACTTACACCAGAGGAATGCCAACAGTTTAAAAAGCAG
  ATAATGAAAGAAATTCAAGAACATAAAATTAAAATATATGAATTTCCAGAAACAGATGATGAAGAAGAGA
  ATAAACTGGTTAAGAAGATAAAGGACCGTTTACCTCTTGCTGTGGTAGGTAGTAATACTATCATTGAAGT
  TAATGGCAAAAGAGTCAGAGGAAGGCAGTATCCTTGGGGTGTGGCTGAAGTTGAAAATGGTGAACATTGT
  GATTTCACAATTCTAAGAAATATGTTGATAAGAACACATATGCAGGACTTGAAAGATGTTACCAATAACG
  TACACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAGTGACTTACAATGGAGTGGATAACAACAAGAA
  CAAAGGGCAGCTTACCAAGAGCCCTCTGGCACAGATGGAAGAAGAAAGAAGGGAACATGTAGCCAAAATG
  AAGAAGATGGAGATGGAGATGGAACAGGTGTTTGAGATGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGG
  ACTCTGAAGCAGAGCTCCAGCGGCGCCATGAGCAAATGAAAAAGAATTTAGAAGCACAGCACAAAGAATT
  AGAGGAAAAACGTCGTCAGTTTGAAGAAGAAAAGGCAAACTGGGAAGCTCAACAGCGTATTTTAGAGCAA
  CAGAACTCTTCAAGAACCTTGGAAAAGAACAAGAAGAAAGGCAAGATCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009859
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009859.4, NP_033989.2
• RefSeq Size: 2533 bp
• RefSeq ORF: 1314 bp
• Locus ID: 235072
• Cytogenetics: 9 A4
• Gene Summary: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]