Arhgap1 (NM_146124) Mouse Untagged Clone

CAT#: MC215871

Arhgap1 (untagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap1
• Synonyms: B230365D05Rik; C76222; Cdc42GAP; p50rhoGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215871 representing NM_146124
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCTCTCTCAGAGCTGCAAGATGACCTGACGCTGGATGACACCAGCCAAGCTCTGAACCAGTTGA
  AGCTGGCCTCCATTGATGAGAAGAACTGGCCTTCTGATGAAATGCCAGACTTCCCCAAGTCAGATGACTC
  CAAAAGCAGCTCTCCAGAACCTGTGACACACCTGAAGTGGGATGACCCTTACTATGACATTGCCCGGCAC
  CAGATTGTGGAAGTAGCCGGTGATGATAAGTATGGGCGGAAGATCATTGTATTCAGTGCTTGCCGGATGC
  CCCCCAGCCACCAGCTGGACCACAGCAAGCTCTTGGGGTATTTGAAACACACATTGGACCAGTATGTGGA
  GAGTGACTACACACTCCTGTACCTGCACCATGGCCTGACCAGTGACAACAAGCCCTCCCTCAGCTGGCTA
  CGGGATGCCTACCGAGAGTTTGACCGCAAGTACAAGAAGAACATCAAGGCTCTGTACATCGTGCACCCAA
  CCATGTTCATCAAAACTCTGCTTATCCTCTTTAAGCCTCTCATCAGTTTCAAGTTTGGACGGAAGATCTT
  CTACGTGAATTACCTGAGTGAGCTGAGCGAGCACGTGAAGCTGGAGCAGTTGGGGATCCCTCGCCAAGTG
  CTCAAGTATGATGACTTCCTCAAATCCACACAGAAAAGCCCTGCAACAGCTCCCAAGCCCATGCCACCAC
  GGCCCCCCCTGCCCAATCAGCAGTTTGGGGTCTCCTTACAGCACCTCCAGGAGAAAAGCCCAGGCCAGGA
  CCCCATTCCCATTGTCCTCAGGGAGACCGTCGCCTACTTGCAGGCTCACGCTCTCACCACTGAGGGCATC
  TTCCGGAGGTCTGCCAACACCCAGGTTGTCCGGGAAGTGCAGCAGAAATACAATATGGGGCTGCCGGTGG
  ACTTTGACCAGTACAACGAGCTGCACTTGCCCGCTGTCATCCTCAAGACATTCCTTCGGGAGCTTCCTGA
  ACCCTTGCTAACCTTTGACCTGTACCCCCATGTTGTGGGCTTCCTCAATATTGATGAAAGCCAGAGAGTG
  GAAGTGACACAGCAGGTTCTCCAAACTCTGCCAGAGGAGAACTACCAGGTGCTTCATTTCCTGACTGCCT
  TCCTGGTGCAGATATCTGCCCACTGTGACCAGAACAAGATGACCAACACTAACTTGGCTGTAGTCTTCGG
  CCCTAACCTGCTCTGGGCTAAAGATGCTGCCATCACCCTCAAGGCTATCAATCCCATCAACACCTTCACT
  AAGTTCCTCCTGGATCATCAAGGGGAATTGTTCCCCAGCACAGATGCCCAGGGGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146124
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146124.4, NP_666236.3
• RefSeq Size: 3113 bp
• RefSeq ORF: 1320 bp
• Locus ID: 228359
• UniProt ID: Q5FWK3
• Cytogenetics: 2 E1
• Gene Summary: GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' structure, resulting in a novel 5' UTR and the use of a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 6 encode the same isoform.