Tbc1d10c (NM_178650) Mouse Untagged Clone

CAT#: MC215855

Tbc1d10c (untagged) - Mouse TBC1 domain family, member 10c (Tbc1d10c), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d10c
• Synonyms: 1810062O14Rik; AI428527
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215855 representing NM_178650
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGCCCTGGGAGAGGACTTGTTATCAGAACTACAGGATGATTCTAGCTCCCTAGGGTCTGACT
  CAGAGCTGAGTGGGCCCAGTCCATATCGCCAGGCTGACCGTTATGGCTTCATTGGGGGCAACTCAGGTGA
  ACTGAGGCTTTGTCAACCCTCTGCAGACCTCATCCGCCAGCGAGAGATGAAATGGGTGGAAATGACCTTA
  CACTGGGAGAAGACCATGTCCCGACGATACAAGAAGGTAAAGATCCAGTGCCGCAAGGGCATCCCCTCAG
  CCCTGCGTGCCCGGTGTTGGCCCCTCCTGTGTGGGGCCCGGATGTGTCAGAAGAACAACCCTGGCACCTA
  CCAGGAGCTGGCAGCAGCCCCCGGGGACCCGCAGTGGATGGAGACCATTGGCAGAGACTTGCACCGTCAG
  TTTCCTCTGCATGAGATGTTTGTGTCACCCCAGGGACACGGGCAGCAGGGGCTGCTGCAGGTTCTCAAGG
  CCTACACCCTGTACCGGCCAGAGCAGGGATACTGCCAGGCTCAGGGACCTGTAGCTGCTGTGCTGCTCAT
  GCATCTACCCCCAGAGGAGGCCTTCTGGTGCTTGGTGCAGATCTGCGAGGTCTACCTCCCTGGCTACTAT
  GGGCCCCACATGGAGGCCGTACAGCTGGATGCTGAAGTGTTCATGGCCCTTCTGAGGCGGCAGCTCCCAC
  GAGTGTACAAGCACCTGCAGCAGGTAGGCGTCGGCCCCCTGCTCTACCTGCCTGAGTGGTTCCTGTGCCT
  CTTCACTCGGTCGCTGCCCTTCCCCACAGTACTGCGCATCTGGGATGCCTTCCTCAGTGAGGGGGCTAAG
  GTACTGTTCCGCGTGGGGCTGACACTGATGCGCCTGGCACTGGGCACTGTAGAACAGCGCACAGCATGCC
  CAGGGCTCCTGGAGACATTGGGGGCCCTACGAGCCATTCCCCCTACCCAGCTGCAGGAAGAGGTCTTCAT
  GTCCCAGGTGCACAGTGTAACCCTGTCTGAGCGAGTCCTGCAACAGGAAATCAGGATCCAGCTAGCCCAG
  CTTTCGAAGTCACTCCCTGGGCCAGCCCCTCTGCCACAGGCCCGCCTTCCTGGGGCCCAAGCCATCTTCG
  AGTCCCAGCAGCTGGCAGGGGTTCGAGAAAGCACCAAACCTGAGATACCTCGAATTGTGGTGCAGCCTCC
  AGAGGAGCCCAAGCCACCACGGCGCAAGCCACAGACCCGGGGTAAGACTTTCCATGGACTCCTGATAAGG
  GCCAGGGGACCCCCTATTGAGGGCCCCTCCAGGTCCCAACGAGGCTCTGCCTCTTTCCTGGACACCCGAT
  TCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178650
• Insert Size: 1335 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178650.3, NP_848765.2
• RefSeq Size: 1781 bp
• RefSeq ORF: 1335 bp
• Locus ID: 108995
• UniProt ID: Q8C9V1
• Cytogenetics: 19 A
• Gene Summary: Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras (By similarity).[UniProtKB/Swiss-Prot Function]