P3h4 (NM_176830) Mouse Untagged Clone

CAT#: MC215830

Leprel4 (untagged) - Mouse leprecan-like 4 (Leprel4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P3h4
• Synonyms: 1110036O03Rik; AI413214; Leprel4; Sc65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215830 representing NM_176830
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGCGGGGCGTGGGGGCTGCTGTGGCTGCTGCTGGGCAGCGCCGGGGCCCAGTACGAGAAATACA
  GCTTCCGAGGATTCCCTCCGGAGGACCTGATGCCACTGGCCACGGCCTACGGCCACGCTCTGGAGCAATA
  CGAAGGCGAAAGCTGGCGCGAGAGCGCGCGCTACCTGGAGGCGGCGCTGCGGCTGCACCGGCTGCTGCGC
  GACAGCGAGGCCTTCTGCCACGCCAACTGTAGTGGCCCCGCCACCTCTCAGCCTCGGCCCGCGTCCGGCC
  CGGACGGCGACCACGACGGCGATGGCGAGGACTGGGCTCGCGAGCTGCGGCTCTTCGGTCACGTCCTGGA
  GCGCGCCGCCTGCCTGCGGCGCTGCAAGAAGACGCTGCCCGCCTTTCAAGTCCCTTACCCACCGCGGCAG
  CTGCTGCGCGACTTCCAGAGCCGCCTGCCCTATCAGTACCTGCACTACGCGCACTTCAAGGCGAACCGAC
  TGGAGAAGGCGGTGGCTGCGGCCTACACCTTCCTCCAGAGGAACCCCAAGCACGAGCTGACCGCCAAGTA
  TCTCAACTACTACAGGGGGATGCTGGATATTGGAGATGAGTCCCTCACCGACCTGGAGGCCCAGCCCTAC
  GAGGCTGTGTTCCTCCGGGCTGTGAAACTTTACAACAGCGGGGACTTTCGCAGCAGCACAGAAGACATGG
  AGCGGGCCCTGGCTGAATATATGACGGTCTTTGCTCGGTGTCTAGCTGGCTGTGAGGGGGCCCACGAGCA
  GGTAGACTTCAAGGACTTCTACCCAGCGATAGCAGATCTCTTCGCAGAATCCCTGCAGTGCAAGGTGGAC
  TGTGAGACCAATCTCACCCCCAACGTAGGTGGCTTCTTTGTGGACAAGTTTGTGGCCACCATGTATCACT
  ACCTGCAGTTTGCCTACTACAAACTGAATGATGTACGCCAGGCTGCCCGCAGTGCTGCTAGCTACATGCT
  CTTTGACCCCAAGGACAGCGTTATGCAGCAGAACCTGGTATATTACCGCTTCCACCGGGCTCGCTGGGGC
  CTTGAAGACGAGGACTTTCAACCCCGGGAGGAAGCTATGCTCTACCACAACCAGACCTCTGAGCTCCGGG
  AGTTGCTAGACTTCACACACATGTACCTTCAGTCAGACGATGAGATGGAACTGGAGGAGACAGAATCACT
  CCCAGAGCCTGAAGAACCTCTATCTGATGCTGAGTTCGAAGGCGAGGGCGACTATGAGGAAGGTCTCTAT
  GCTGACTGGTGGCAAGAGCCAGAAGCCAAGGGTGACGAGGCCGAGGCCGAGCCAGAGCCTGAACTGACAT
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176830
• Insert Size: 1332 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176830.2, NP_789800.1
• RefSeq Size: 1903 bp
• RefSeq ORF: 1332 bp
• Locus ID: 66180
• UniProt ID: Q8K2B0
• Cytogenetics: 11 D
• Gene Summary: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils (PubMed:27119146). Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:23959653, PubMed:27119146, PubMed:28115524).[UniProtKB/Swiss-Prot Function]