Eral1 (NM_022313) Mouse Untagged Clone

CAT#: MC215823

Eral1 (untagged) - Mouse Era (G-protein)-like 1 (E. coli) (Eral1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eral1
• Synonyms: 2610524P08Rik; 9130407C09Rik; AU019798; Era; M-ERA; MERA-S; MERA-W
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215823 representing NM_022313
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCCCAGGCGGTACTGCGCTGGGCTCGTTCGGGCCTTGTTAGGGGCCCGACAGGTGGGTTCGC
  ATGCAGGGAGGGAATGGCTTGCTCCGCCCGGCTGTCTTCTAGGTAACCAGGCGAGGTGCGTGTCCTGTGT
  CGTGGGCTCCACTTTCTCTGGTCCCCTCCTGGCCTCGGCCTCTAGTCGTTATGGTCAGGACTCCGCCTTG
  GACCGTATCCTTGGATTTTCTCAGCCTGACAGTTCGTTGGTTCCGAGCGTCCCCGCGGTGTCGGTGCACA
  GAGATGAGCAGAATCTACTCTTGGTGCACACTCCCGACATGCCTGAGAATCCACGGGTCCTCCGCGTGGT
  CCTCCTGGGAGCCCCAAATGCAGGGAAGTCAACACTTTCCAATCAGCTGCTGGGCCGTAAAGTGTTTCCT
  GTTTCAAAGAAGGTGCACACCACTCGATGTCAAGCTCTGGGGGTCATCACAGAGAAGGAGACCCAAGTGA
  TTCTGCTTGACACACCTGGCATCATCAGTCCTGTTAAACAGAAGAGGCACCATCTGGAGCGCTCTTTGCT
  GGAAGATCCATGGACGAGCATGGAATCTGCTGATCTTGTTGTAGTTCTTGTGGATGTGTCAGACAAGTGG
  ACCAGGAGTCGGCTCAACCCCCAGGTGCTGCAGTGCTTGACCAAGTTCTCTCAGGTCCCTAGCATCCTCG
  TCTTAAACAAGGTAGATTGCCTGAAGCAGAAGTCAGTTCTCCTGGAGCTGACAGCAGCCCTCACTGAAGG
  TGTTGTCAATGGCAAGAAGCTTAATATCAAGCAGGCCTTGCGCTCACGCTCCAGCACCCACTGCCCTGGC
  CCTGAAACTGAGGGCCCAAATGCACACTCAGTGAGGAACCCTCAGAGAATTGGCTGGCCCTACTTCCAGG
  AGATCTTCATGTTATCAGCACTAAACAACAAGGATGTGAACACATTGAAGCAATACCTTCTGACACAGGC
  CCAGCCAGGACCCTGGGAGTTCCACAGTGGTGTGCTCACTAGCCAGACACCCGAAGAGATCTGTGCCAAT
  AAAATCAGAGAGAAGCTCTTAGAGTACCTGCCTGAGGAGGTGCCCTACGGTGTGCAGCAGAAGACAGTGA
  TATGGGAGGAAGGACCAAGTGGAGAGCTAGTGATCCAACAGAACCTTCTGGTGCCCAAAGAATCTCATGT
  GAGGATCCTGATTGGTCAGAAGGGCCTCTTGATCTCCCAGATTGCACAGGAGGTGGGCCGGGACCTCATG
  GACATCTTCCACTGTGACGTTCTCATCCGCCTCTCTGTGAAACTCCTCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022313
• Insert Size: 1314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022313.2, NP_071708.2
• RefSeq Size: 2055 bp
• RefSeq ORF: 1314 bp
• Locus ID: 57837
• UniProt ID: Q9CZU4
• Cytogenetics: 11 B5
• Gene Summary: Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly (By similarity).[UniProtKB/Swiss-Prot Function]