Pus1 (NM_001025561) Mouse Untagged Clone

CAT#: MC215822

Pus1 (untagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pus1
• Synonyms: A730013B20Rik; MPUS1; mPus1p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215822 representing NM_001025561
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAAACTGGGGACGGTGGACGGCGCGCCCAGGCCCGC
  GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGGCCTCACACCAACCGGACAG
  GAAGGGCCGAGGTGGCTGGGTCTGGGAGGAGACGGAGCATCCTGCAAAGAGGGTCAAGGGCGGCGAGGAT
  GAAGAGCCCCCACGCAAGCTGCCAAAGCGAAAAATTGTGCTGCTCATGGCCTACTCGGGCAAGGGCTACC
  ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCGGCCTTAGT
  CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCCTTCCAGCGGTGTGCTCGA
  ACAGACAAGCCTTTGAGGACCTCTGCCTGCTTTTGCCTGTACACTGGCTTCCTCTTCCACTGGGGTGTGT
  CTGCAGCAGGGCAGGTGGTATCCCTAAAGGTGTGGCTGATTGATGACATTCTGGACAAGATCAACAGCCA
  CCTTCCATCCCACATTCGGATTCTGGGATTGAAGAGGGTCACAGGCGGGTTCAACTCCAAGAACAAGTGT
  GACGCCAGGACCTACTGCTACATGCTGCCCACCTTTGCCTTTGCTCACAAGGACCGGGATGTACAGGACG
  AGAGCTATCGCCTGAGTGCAGAGACCCTGCAGCAGGTCAACCGCCTTCTGGCCTGCTACAAAGGCACCCA
  TAACTTCCATAACTTCACCTCGCAGAAGGGCCCAAGAGAGCCCAGTGCGCGCCGCTACATTCTGGAGATG
  TACTGCGAAGAGCCCTTCGTACGTGAGGGCCTGGAGTTTGCTGTGATCAAGGTGAAGGGCCAGAGCTTCA
  TGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTGAAGGGTTATGCCCCTGAGAGTGT
  CCTGGAGCGCAGCTGGGGAGAGGAGAAGGTGGATGTGCCCAAGGCACCCGGGCTTGGCTTAGTCCTGGAG
  AGGGTTCACTTTGAAAAGTACAATCAACGCTTTGGCGGTGACGGGCTGCATGAGCCCCTGGACTGGACGC
  AGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAGTACATTTACCCTACCATTGTCAGCACTGAGCGGGA
  TGAGCGGTCCATGGCCCAGTGGCTCAATACTTTGCCGATCCACAACTTCAGTGGCACTGCACTTGGAGCG
  GCTGACACAGGTGCCAAGGTCCCCAGTTCCCTGGAGGGCAGTGAAGGGGATGGAGACACTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025561
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025561.3, NP_001020732.1
• RefSeq Size: 1868 bp
• RefSeq ORF: 1326 bp
• Locus ID: 56361
• Cytogenetics: 5 F
• Gene Summary: Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:10094309). Involved in regulation of nuclear receptor activity possibly through pseudouridylation of SRA1 RNA (PubMed:15327771). Isoforms 3 and 4 do not form pseudouridine when expressed in vitro (PubMed:11085940).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.