Nim1k (NM_175538) Mouse Untagged Clone

CAT#: MC215756

Nim1 (untagged) - Mouse RIKEN cDNA E130304F04 gene (E130304F04Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nim1k
• Synonyms: E130304F04Rik; Nim1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215756 representing NM_175538
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAGTGTTTGTGAATGGAGGCGGCCTGGTGAACACACATTGTGACAGGTGGGAGCGGAGGGACA
  GCGTGGAAAGCAGCTGTCAGACCGAGGGCGGCAAGGACAGCGAGGAGGACCAGCAGCGCCAGCTGACACC
  CTTCGAGAAACTGACTCAGGACATGTGCCAGGATGAGAAGGTGGTGAGGGAGATCACGCTGGGGAAACGC
  ATTGGCTTCTATCGGATTCGAGGGGAGATCGGAAGCGGAAACTTTTCCCAGGTCAAGCTGGGGATTCACT
  CCCTAACCAAAGAAAAGGTGGCCATTAAGATTCTGGACAAAACCAAGTTAGACCAGAAAACCCAAAGGCT
  GTTATCCAGAGAGATTTCCAGCATGGAAAAGTTGCATCATCCCAACATCGTCCGTCTTTATGAAGTCGTG
  GAGACACTGTCCAAGCTACACCTAGTGATGGAGTATGCAGGAGGTGGGGAGCTTTTTGGAAAAATTAGCA
  CCGAGGGGAAGCTTTCTGAACCGGAAAGCAAGCTTATCTTCTCCCAGATCCTGTCTGCCGTGAAGCACAT
  GCATGAAAATCAAATTATTCACAGAGATCTGAAAGCAGAAAACGTCTTTTATACCAGTAGGACTTGTGTG
  AAGGTGGGAGATTTTGGATTCAGCACAGTAAGTAAGAAAGGCGAGATGCTGAACACTTTCTGTGGATCTC
  CACCCTACGCGGCACCAGAACTCTTTCGAGACCAACACTACGTTGGTGTTTATGTGGATATCTGGGCCTT
  GGGTGTCCTTTTGTACTTCATGGTGACTGGCACGATGCCATTTCGAGCGGAGACCGTGGCCAAGCTAAAG
  AAGAGCATCCTCGAGGGTACCTACACCATACCCCAGCATGTGTCTGAGCCTTGTCACCGGCTCATCCGAG
  GAGTCCTTCAGCCCACGCCCACAGAGAGGTATGGGATCAACTACATCATGAGCAATGAATGGATGCGAGG
  GGTGCCATACCCCACCCCTTTGGAACCGTTCCAACTGGATCCCAAACATTTGTCGGAAACCAGTACCCTC
  AAAGAAGAAGAAAACGAGGTGAAGAGCACTTTAGAACACTTGGGGATTACAGACGAACATATCCGGAATA
  ACCAAGGGAGAGATGCTCGGAGCTCTATCACGGGGGTCTATAGAATCATTTTACATCGAGTGCAAAGAAG
  AAAAGCATTGGAAAGTGTGCCAATAATGATACTACCAGAACCTAAAGAAAGGGACTTAAAGAAAGGGTCC
  CGTATCTACAGAGGCATAAGACACACATCTAAATTTTGTTCAATTTTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175538
• Insert Size: 1311 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175538.3, NP_780747.1
• RefSeq Size: 4103 bp
• RefSeq ORF: 1311 bp
• Locus ID: 245269
• UniProt ID: Q8BHI9
• Cytogenetics: 13 D2.3