Meikin (NM_029105) Mouse Untagged Clone

CAT#: MC215737

4930404A10Rik (untagged) - Mouse RIKEN cDNA 4930404A10 gene (4930404A10Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Meikin
• Synonyms: 4930404A10Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215737 representing NM_029105
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGATATGGCATATGGGGCCCAGAGGGGACTATACCCGCAAGAAGAGGGCTGGTGAAAGGCTCA
  ACCTCACCCCGAAGCCAGACCTGGCCCTCCCTGGGAGGACAGAGGCCCTGCCAGGTCTGAAGGGAAAGGG
  CAAAGAGCAGGGATTGCGGAAAATTACTGAGAAGAAGGAATTGAGCAGACTCACAGGTAGCAGCTCACAG
  CGGCCAAGTCTGTTAAGTGTTACAGGAGGGGAACACCTGCAAGAAAATAGCCCTGGCCAAGAGACCCCAG
  AGGAAAAGACACCACCATGTGAAACAGTGACTGATACCTTTGAGATGGACAGCTTGTTGTCGAGTACTGA
  GCTAGTTTCAGGACCAGCCGAGCAGGATGACTTTTCCAGCTGTCTCCCGAGTTGTTCAAATGCAGAGTTA
  CATACAGAGAGCACCGATGAGAGAGGAAGTAGCTTCCCATCGCCAGAACTGTTCAGAGGGTCAGACTGCC
  TGGATTGGGAGCATCCCAAGTTGGAAGATTACATGTTCTACAAGAATTCTACTCTTTTAGACACCAGTAA
  AGCAGTAGTTATAGAGAAAGCGCCACAGTTTGCAAACCTCTCTGCAGTTCTGAGTTCCTCTTCAAAAAAC
  TATGAGAAACGCCATAGAAAAATAGGGATGACATTAGCAGCCCAACATCTTTCTCCAGAACCAAAGTATG
  CTTCAAATTTAGCATCAGTTGTAGATAATGCAGCTTCTGAGGTTGTATTTGCTGAGAAAACTGGCCCTCC
  AACCACTGAAAAAACACAGAAAAAGCCTGAAAATGAATCTGAAGATAGTGGTCCGCTCGTTCAAACAAAG
  TTGAGCTCCGGTCATCCAGACAACAAGGCTCTGTGCTCCCCTCTAAGTTCAGCTCTTGAAAGCACTGCAG
  TCAGGTATACTCTGCTGCCTCAGCCCCTGGAACCTGTGCTGAAAAAAGGTTGTATTCTTCCTGATAAACA
  AAGCAAAGCCCTGCTAACAAGTACCCCATCTTCAGACATAGCTGAGTTTGTGATTGATTTGTCTCCAGTG
  CAAAATGTGTCTTTTGAAGAACTATTTCCAAATGTCAGCAATTATGTTAACTCAAGTGAAGTTGTTCCTG
  TGTCAAGTTTGCAAGAAAGTTCATCAAATGAGTTTTCTCCAAACACATCAGAGATCTGTTGTATTATTAG
  GTCATCACCAGGAACCAGACAGATGAGAAGGAAAGACCCTGCTGTGAAAAACAGATGTTCTCCTCCTAAA
  GATGTTCCTCTAGATATTATAATGAAGACAAATGGCAGAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029105
• Insert Size: 1305 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029105.2, NP_083381.1
• RefSeq Size: 1433 bp
• RefSeq ORF: 1305 bp
• Locus ID: 74847
• UniProt ID: Q5F2C3
• Cytogenetics: 11 B1.3
• Gene Summary: Key regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage. Acts by facilitating kinetochore mono-orientation during meiosis I, when kinetochores on sister chromosomes face the same direction and are thus captured and pulled by spindle fibers from the same pole. Also required to prevent cleavage of cohesin at centromeres during meiosis I, possibly by acting as a regulator of the shugoshin-dependent protection pathway. Acts in collaboration with PLK1: required for PLK1 enrichment to kinetochores. Not required during meiosis II or mitosis.[UniProtKB/Swiss-Prot Function]