Clnk (NM_013748) Mouse Untagged Clone

CAT#: MC215725

Clnk (untagged) - Mouse cytokine-dependent hematopoietic cell linker (Clnk), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clnk
• Synonyms: MIST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215725 representing NM_013748
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGCCAGGGCAATAAAAGGACAACGAAAGAAGGATTCGGTGATCTGAGATTCCAGAACGTCTCTC
  TGCTGAAAAATAGGTCATGGCCAAGCCTCAGCAGTGCCAAAGGGCGGTGTCGAGCGGTTCTGGAACCACT
  TCCGGATCACAGAAGGAACTTGGCTGGGGTCCCAGGTGGAGAAAAATGCAACAGTAACAACGACTACGAA
  GATCCTGAGTTCCAGCTGCTGAAGGCATGGCCATCAATGAAAATTTTACCAGCCAGACCTATCCAGGAAT
  CGGAATACGCAGATACACGCTATTTCCAGGATACGATGGAGGCTCCCCTTCTGTTACCTCCCAAGGCTTC
  TGTCTCCACTGAGAGACAAACCAGGGATGTGAGGATGACACATCTGGAAGAAGTGGACAAGCCTACCTTC
  AAGGATGTCAGAAGCCAACGCTTTAAAGGATTCAAATACACAAAAATAAACAAGACTCCTTTGCCACCTC
  CTCGGCCTGCTATCACTCTCCCCAAGAAGTACCAACCCTTACCCCCAGCACCACCAGAGGAGAGCAGTGC
  ATACTTCGCTCCAAAGCCCACCTTTCCAGAAGTCCAGAGGGGGCCCAGGCAGAGGAGTGCAAAAGACTTC
  AGTAGGGTCCTTGGAGCAGAAGAAGAATCTCACCACCAGACAAAGCCAGAATCTTCTTGCCCATCATCAA
  ACCAAAACACACAGAAGAGTCCACCTGCCATTGCCAGCTCTTCCTACATGCCAGGAAAGCACAGTATACA
  AGCCAGAGACCATACAGGTAGCATGCAGCACTGTCCTGCTCAGAGATGCCAAGCTGCAGCCAGCCACAGC
  CCTCGAATGCTGCCCTATGAAAACACAAACTCGGAGAAACCTGACCCCACAAAGCCTGATGAGAAGGATG
  TCTGGCAGAATGAATGGTACATTGGAGAATACAGTCGCCAGGCAGTGGAAGATGTGTTAATGAAAGAGAA
  CAAGGATGGTACTTTTTTGGTCCGAGACTGCTCTACAAAATCCAAGGCAGAACCATATGTTTTGGTGGTG
  TTTTATGGGAACAAGGTCTACAATGTGAAAATCCGTTTCCTCGAGAGCAATCAACAGTTTGCCCTGGGCA
  CAGGACTACGAGGAAATGAGATGTTTGATTCTGTGGAAGACATCATTGAACACTACACATATTTTCCCAT
  TCTGCTAATAGATGGGAAAGACAAGGCTGCACGCAGGAAACAGTGCTACCTCACCCAGCCACTGCCTCTC
  GCCAGGCTCCTTCTCACTCAGTACTCCAGCCAGGCACTTCATGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013748
• Insert Size: 1308 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013748.3, NP_038776.3
• RefSeq Size: 1586 bp
• RefSeq ORF: 1308 bp
• Locus ID: 27278
• UniProt ID: Q9QZE2
• Cytogenetics: 5 B3
• Gene Summary: Plays a role in the regulation of immunoreceptor signaling, including PLC-gamma-mediated B-cell antigen receptor (BCR) signaling and FC-epsilon R1-mediated mast cell degranulation. Involved in phosphorylation of LAT.[UniProtKB/Swiss-Prot Function]