LOC100048775 (NM_001101423) Mouse Untagged Clone

CAT#: MC215651

LOC100048775 (untagged) - Mouse middle T antigen (LOC100048775), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: LOC100048775
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215651 representing NM_001101423
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGAGTTCTGAGCAGAGCAGACAAAGAAAGGCTGCTAGAACTTCTAAAACTTCCCAGACAGCTAT
  GGGGGGATTTTGGAAGAATGCAGCAGGCATATAAGCAGCAGTCACTGCTACTGCACCCAGACAAAGGTGG
  AAGTCATGCTTTAATGCAGGAATTGAACAGTCTCTGGGGAACATTTAAAACTGAAGTATACAATCTGAGA
  ATGAATCTAGGAGGAACCGGCTTCCAGGTAAGAAGGCTACATGCGGATGGGTGGAATCTAAGTACCAAAG
  ACACCTTTGGTGATAGATACTACCAGCAGTTCTGCAGAATGCCTCTTACCTGCCTAGTAAATGTTAAATA
  CAGCTCATGTAGTTGTATATTATGCCTGCTTAGAAAGCAACATAGAGAGCTCAAAGACAAATGTGATGCC
  AGGTGCCTAGTGCTTGGAGAGTGTTTTTGTCTTGAATGTTACATGCAATGGTTTGGAACACCAACCCGAG
  ATGTGCTGAACCTGTATGCAGACTTCATTGCAAGCATGCCTATAGACTGGCTGGACCTGGATGTGCACAG
  CGTGTATAATCCAAGGCTCTCCCCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001101423
• Insert Size: 588 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001101423.1, NP_001094893.1
• RefSeq Size: 588 bp
• RefSeq ORF: 588 bp
• Locus ID: 100048775
• Gene Summary: DISCONTINUED: This record has been withdrawn by NCBI staff. This record was defined by X59849.1, which represents a polyoma virus-related mammalian plasmid sequence and is out of scope for Gene.