Acd (NM_001012638) Mouse Untagged Clone

CAT#: MC215064

Acd (untagged) - Mouse adrenocortical dysplasia (Acd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215064 representing NM_001012638
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGATTCAGGGTTGCTGGCCCTACAGCCCTGGATTCGAGAGCTGATCCTAGGTTCAGAAACACTGT
  CAAGTCCACGAACCGGGCAGCTGCTCAAGGTACTCCAGGACTCGGAGACTCCCGGCCCTTCTTCCGCCCC
  TGACACACCTGACACCGGGGCCGTGTTACTTGTGTCAGACGGAACCCACAGTGTCCGATGCGTGGTGACG
  CGCAACGCCATCGACACCTCCGACTGGGAGGAGAAGGAGCTCGGATTCCGCGGGACCGAGGGCCGGCTCC
  TGCTACTGCAGGCCTGCGGGCTCCGCGTTCAGGTGGCTCAGGACCATGCGCCTGCGGAGTTCTACCTCCA
  GGTGGATCGCTTTAACCTGCTGCCCACGGAGCAGCCCCGGATACAGGTGACTGGTTGCAATCAGGATTCA
  GATGTGCAGAGAAAACTCAATGAGTGTCTTGAGGACCACCTCTCAGAGTCTGCTTCTTCCAGTGCAGGCC
  TAACCCTGTCCCAGCTTCTGGATGAGGTGAGGGAGGACCAGGATCATCGGGGAGCCCTAGTGTGTCTAGC
  TAAGAGCTGCCTGGTGCTGAAAGGTCCTTGTACAACCACCCCCCTCACCGACTGGATCACCTCTGGCTCC
  CAGGCCTTGGGAAAAGCTGTGTTCACTGTGTCTGGCTCATTGCTGCACATCCCTGAGGGTGAGGAGCAGA
  TTTTGAGTTCTACAGGCTCCAGTCAGAAGGCTCGGGGAACCTCCGCTTCACCCAGCCACATGCCCCTGGA
  GGAAAGTGGTGCCAGCGTTAGCCTTCTGTCAGCCTTGGCTACATCGGACCCTGGGCAGATGGACAGTTCC
  CAGTCTCCACCAGCTGTTGGCTCAACCTCCCCAAGAGCTCAGGCCCCCACTTCTCCACCCTGCAATTCTA
  CACCCAGCTCCCTACTCTTGAACTGCAGCCCCAGTCTTTCACCCCTTCACCCTGCCCCTAGATCACACCA
  GTCCTGTGAGACCAGAGCTCAGGCTCCTAAGCTGGAGTTCCAGTGCTCCTTCAAGAAACGGCAGCTTCTC
  CCAAGAACCAGCGCCCAGGAGCTCTGCTCTGTGTGGGAACCCCCAGAGAGGCATCGTGATACTTCTGCAT
  TCCAGTATAAATACGAGACACCCTCTGCCTCCCTCCACACCCAGGTCCAAACTGCCAGGCTCTCTCCTCA
  GCTTGTAGCTTGGGCCTTGAATATAGTGATGGAGTCAGAGTCTGAGTTAACCCAGGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012638
• Insert Size: 1251 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC138694, AAI38695
• RefSeq Size: 1425 bp
• RefSeq ORF: 1251 bp
• Locus ID: 497652
• UniProt ID: Q5EE38
• Cytogenetics: 8 53.04 cM
• Gene Summary: Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity (By similarity). May play a role in organogenesis (PubMed:15537664).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).