Spag8 (NM_001007463) Mouse Untagged Clone

CAT#: MC214976

Spag8 (untagged) - Mouse sperm associated antigen 8 (Spag8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spag8
• Synonyms: MH-SPAG8; SMP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214976 representing NM_001007463
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCACCGAGTCTACAGAGGGGTCTTTGTCGCGATCTTGTGATGTACAGCCCAGCTCCGAAAGAC
  TAGATACCCCTTCAGAACCTGTTCCTTCTTCGAGTAGTAGTCCTAGGTCTACTGCCCCTGCCGAGGCTCC
  GGCGCAGTACTCTGTGCTGACAGAGCCCTCTTCTGACAGTCTTTACGGGGCACCCTGCCCTCCAGCCCAT
  CATAGAGGCCATGGGTTTGGCTTTCAGCCCTTCTATGTTTCTTGTATTCCTCAGGATCCCTGTAATATGG
  CAGACCTCAGCTCCAGGGCTGATCCTACCTCTTCTTATCCTTGTCATAGCTCTGTGCATGGCTCTGGCTC
  TGGCACTTGTGGTCTTGGCCAGAGCTCTGAGCCTAGCCAGGGCTCCGGGCCCACCTCTGGGCCTGCTCCT
  GCCTCTGTACCTAGTCTTGTCTCTGGACCTGATTCTGCCTCTGGACCTGATTCTTCTGCCTCGGGACCTG
  CTCTTGCCTCTGGACCTGGTCCTGCTGACCCTGGACAGGGTCCTAAGTTCAGCACCTGCATTCCTCAAGG
  GTACAGATGCATCCCAGTTGACCTGGCCCCTGATTATAATGCATGGTGTCAGCACTTACACTGGAAGCCA
  CAACGCTCCTGGGAACCTCTGCAAGTCTCAGAACCTGGAGTGAGAGGGCCATATAAACCTCCAGAACCTG
  GAGCACTCGGGCCATGTGAGCCATGTGAGCCATGTGAACCTCCAGAGGCTGAATCTGAGGAAACTCTCTG
  TAAAGCACGGCCACGTGGCCAGTGCCTCCTCTACAACTGGGAGGAAGAGAGAGCCACCAACCAGCTGGAT
  CAAATCCCTCCATTGCAAGATGGCTCTGAGAGTTACTTCTTCCGACACGGACACCAGGGTCTGCTGACCA
  CGCAGCCCCAGTCACCCATGTCTTCTAGCACCACCCAGAGAGACTCATACCAGCTCCCAAGACACATCTG
  TCAGCCACTTCGAGGGAAGCGTGAAGCCATGCTGGAGATGCTCCTGCGACACCAGATCTGTAAAGAGGTG
  CAGGCAGAGCAGGAACCTGCAAGGAAGCTCTTTGAGACCGAGTCGGTAACACACCATGACTACAGAGTGG
  AGCTGGTACGAGCAGCGCCTCCTGCCTCAACAAAGCCTCATGATTACCGCCAGGAGCAGCCTGAAACCTT
  CTGGATTCAGCGGGCAGCACGGCTACCGGTGTGTGTGGGCGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007463
• Insert Size: 1236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007463.2, NP_001007464.1
• RefSeq Size: 1342 bp
• RefSeq ORF: 1236 bp
• Locus ID: 433700
• UniProt ID: Q3V0Q6
• Cytogenetics: 4 A5
• Gene Summary: Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (PubMed:20488182). Involved in the acrosome reaction and in binding of sperm to the zona pellucida (PubMed:17187156). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis (By similarity). May play a role in fertility and microtubule formation through interaction with RANBP9 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.