Neil2 (NM_201610) Mouse Untagged Clone

CAT#: MC214776

Neil2 (untagged) - Mouse nei like 2 (E. coli) (Neil2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Neil2
• Synonyms: Gm1212; NEH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214776 representing NM_201610
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGAAGGGCCATCTGTGAGGAAGTTTCACCATCTTGTCTCCCCCTTTGTGGGCCAGAAGGTGGTCA
  AGACGGGGGGCAGCAGTAAGAAGCTCCACCCTGCCGCCTTTCAGTCTCTGTGGCTCCAGGATGCTCAGGT
  GCATGGAAAAAAATTATTCCTTCGGTTTGATCCAGATGAGGAGATGGAGCCACTCAACAGCAGCCCACAG
  CCTATACAGGGAATGTGGCAGAAAGAGGCTGTGGACCGAGAGCTGGCCTTGGGTCCCAGTGCTCAGGAAC
  CCTCTGCAGGTCCCTCTGGATCTGGGGAGCCTGTTCCCAGTAGATCTGCTGAAACATATAATCTTGGGAA
  GATCCCCTCAGCAGATGCCCAGAGGTGGCTGGAGGTCAGGTTTGGTTTATTTGGCAGTATCTGGGTGAAT
  GACTTCTCCAGAGCAAAGAAAGCTAACAAAAAAGGTGACTGGAGAGACCCAGTGCCCAGGCTGGTACTCC
  ATTTTAGTGGTGGTGGCTTCCTGGTATTTTATAACTGCCAGATGTCATGGAGCCCTCCCCCAGTGATTGA
  GCCCACCTGTGACATATTGTCTGAAAAGTTCCATCGAGGACAAGCCTTGGAAGCTCTAAGCCAGGCTCAG
  CCTGTGTGCTACACACTCTTGGACCAGAGATACTTCTCAGGATTAGGGAACATCATAAAGAATGAAGCCT
  TGTACAGAGCAAGGATCCATCCCCTCTCTCTCGGTTCATGCCTGAGTTCTTCCTCTCGGGAGGCCCTCGT
  GGATCACGTGGTGGAGTTCAGTAAGGACTGGCTTCGGGACAAATTCCAAGGCAAGGAACGGCACACACAG
  ATCTACCAGAAGGAACAATGTCCTTCTGGTCACCAGGTCATGAAGGAGACATTTGGGCCCCCAGATGGGC
  TCCAGAGGCTCACCTGGTGGTGCCCTCAATGCCAGCCCCAGCTGTCCTCCAAGGGGCCCCAGAATCTCCC
  GTCCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_201610
• Insert Size: 990 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201610.2, NP_963904.2
• RefSeq Size: 1914 bp
• RefSeq ORF: 990 bp
• Locus ID: 382913
• UniProt ID: Q6R2P8
• Cytogenetics: 14 D1
• Gene Summary: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).[UniProtKB/Swiss-Prot Function]