Cracr2a (NM_001033464) Mouse Untagged Clone

CAT#: MC214729

Efcab4b (untagged) - Mouse EF-hand calcium binding domain 4B (Efcab4b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cracr2a
• Synonyms: Efcab4b; Gm462; Gm1073
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214729 representing NM_001033464
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCCCAGTGGAAGAGAAGACTCCAGCTCCCAGACACCAGGTCATGGGAAGCAGGGAAGTGGAG
  CCTGCGTGGAGCAACTGGACCACCCGGAGAAGCTGGAGGTGGAGATGCCAGACCAGAGTGCCATGTGGAA
  GAAAGCACAGGAATTCTTCCAGACCTGTGACTCCGAGGGCAAGGGCTTCATTGCCAGGACAGACATGCAG
  AGGCTGCATCAGGAGTTGCCCCTAAGTCTAGAAGAATTGGAAGATGTGTTTGACGCCCTGGATGCTGATG
  GCAATGGCTTTCTGACCCCAGAAGAGTTCACCACTGGATTTAGTCACTTCTTCTTCAGTCAAAATATCCA
  AGGAGAGGAAGAAGCTGACCAGCAGGTGGCCCAGCTCCAAGAGGAGAAAGTGTATCAGTCTAGAGGGGAG
  GAGGATGTGGGAGACATGGACCATGATGAAGAAGCACAATTTCAGATGCTGATGGACAGACTTGGAGCCC
  AGAAAGTTCTGGAAGATGAAAGTGACGTCAGGCAGCTGTGGCTGCAGCTGAGGAAGGACGAGCCCCACCT
  GCTGTCCAACTTCGAAGACCTCCTCACCACAATTTTTGCTCAGCTCCAAGAAGCCCATGAGCAGAAGAAC
  GAACTGGAATGTGCCCTCAGAAAGAAAATTGCTGCTTACGATGAAGAAATCCAGCATCTCTATGAGGAGA
  TGGAGCAGCAGATCAAAAGTGAGAGGGAGCAGTTCCTCCTGAAAGACACAGAGAGATTCCAGGCTCGCAG
  CCGGGAGTTGGAAAAGAAGCTGAGCGCCAAGGAGCAGGAGCTGGAGCGACTCAATCAGAAGCAGAGGAAG
  GTAGGCTACTGTGGAGACATCGTGGGCCCTCAGCTCTTCCAACTCAGCTTGCCTCTGCCTCACGCCCTCC
  ACCATTCGTCCATGGATTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033464
• Insert Size: 933 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033464.3, NP_001028636.1
• RefSeq Size: 1094 bp
• RefSeq ORF: 933 bp
• Locus ID: 381812
• UniProt ID: Q3UP38
• Cytogenetics: 6 F3
• Gene Summary: Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex (By similarity).[UniProtKB/Swiss-Prot Function]