Ifi211 (NM_001033450) Mouse Untagged Clone

CAT#: MC214701

Mnda (untagged) - Mouse myeloid cell nuclear differentiation antigen (Mnda), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ifi211
• Synonyms: A730048F03; ifi-205-B; Ifi20; Ifi205b; Mn; Mnda
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214701 representing NM_001033450
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAATGAATACAAGAGAATTGTTCTGCTGAGAGGACTTGAATGTATCAATAAGCATTATTTTAGCT
  TATTTAAGTCATTGCTGGCCAGAGATTTAAATCTGGAAAGAGACAACCAAGAGCAATACACCACGATTCA
  GATTGCTAACATGATGGAAGAGAAATTTCCAGCTGATTCTGGATTGGGCAAACTGATTGAGTTTTGTGAA
  GAAGTACCAGCTCTTAGAAAACGAGCTGAAATTCTTAAAAAAGAGAGATCAGAAGTAACAGGAGAAACAT
  CACTGGAAAAAAATGGTCAAGAAGCAGGTCCTGCAACACCTACATCAACTACAAGCCACATGTTAGCATC
  TGAAAGAGGCGAGACTTCTGCAACCCAGGAAGAGACTTCCACAGCTCAGGCGGGGACTTCCACAGCTCAG
  GCGGGGACTTCCACAGCTCAGGCGGGGACTTCCACAGCCCAGAAAAGAAAAAGTATGAGAGAAGAAGAGA
  CTGGAGTGAAAAAGAGCAAGGCGGCTAAGGAACCAGATCAGCCTCCCTGTTGTGAAGAACCCACAGCCAT
  GTGCCAGTCACCAATACTCCACAGCTCATCTTCGGCTTCATCTAACATTCTTTCGGCTAAGAACCAAAAA
  TCACAACCCCAGAACCAGAACATTCCCAGAGGTGCTGTTCTCCACTCAGAGCCCCTGACAGTGATGGTGC
  TCACTGCAACAGACCCGTTTGAATATGAATCACCAGAACATGAAGTAAAGAACATGTTTCATGCTACAGT
  GGCTACAGTGAGCCAGTATTTCCATGTGAAAGTTTTCAACATCGATTTGAAAGAGAAGTTCACAAAAAAT
  AATTTTATCACCATATCCAATTACTTTGAGAGCAAAGGCATCCTGGAGATCAATGAGACTTCCTCTGTGT
  TAGAGGCTGCTCCTAAACAAATGATTGAAGTGCCCAACTGTATTACCAGAAATGCAAATGCCAGTCCTAA
  GATCTGTGATATTCAAAAGGGTACTTCTGGAACAGTGTTCTATGGAGTGTTTACATTACACAAGAAAAAA
  GTGAAAACACAGAACACAAGCTATGAAATAAAAGATGGTTCAGGAAGCATAGAAGTTGTGGGGAGTGGAC
  AATGGCACAACATCAACTGTAAGGAAGGAGATAAGCTCCACCTCTTCTGCTTTCACCTGAAAAGAGAAAG
  AGGACAACCAAAGTTAGTGTGTGGAGACCACAGTTTCGTCAAGGTCACCAAGGCTGGGAAAAAAAAAGAA
  GCATCAACTGTCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033450
• Insert Size: 1278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033450.4, NP_001028622.1
• RefSeq Size: 1789 bp
• RefSeq ORF: 1278 bp
• Locus ID: 381308
• UniProt ID: P0DOV1
• Cytogenetics: 1 H3
• Gene Summary: The protein encoded by this gene is a member of the interferon-regulated 200 family of proteins, which contain an N-terminal pyrin domain that is proposed to function in cell death and a partially conserved 220 amino acid domain. Expression of this protein in embryonic stem cells is critical for the DNA damage response and regulation of cell survival. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.