Nanos2 (NM_194064) Mouse Untagged Clone

CAT#: MC214663

Nanos2 (untagged) - Mouse nanos homolog 2 (Drosophila) (Nanos2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nanos2
• Synonyms: nos2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214663 representing NM_194064
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTACCGCCCTTTGACATGTGGAGAGACTACTTTAACCTGAGCCAGGTGGTGATGGATATAATTC
  AGAGCCGGAAGCAAAGACAGGAGGGTGAGGTAGCTGAGGAGCCCAACTCCAGGCCCCAGGAGAAGAGTGA
  GCAGGACCTGGAGGGCTACCCTGGATGTCTGCCTACCATATGCAACTTCTGCAAGCACAATGGGGAGTCT
  CGTCACGTCTACACCTCACACCAGCTGAAGACGCCTGAAGGGGTGGTTGTGTGTCCCATCCTGAGGCACT
  ATGTGTGTCCTCTATGTGGAGCCACCGGCGACCAGGCTCATACACTCAAGTATTGTCCACTCAACAGCAG
  TCAGCAGTCTCTCTACCGACGCAGTGGGCGAAACTCAGCTGGTCGCAGAGTCAAGCGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_194064
• Insert Size: 411 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194064.2, NP_918953.2
• RefSeq Size: 1439 bp
• RefSeq ORF: 411 bp
• Locus ID: 378430
• UniProt ID: P60322
• Cytogenetics: 7 A3
• Gene Summary: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.[UniProtKB/Swiss-Prot Function]