Ust (NM_177387) Mouse Untagged Clone

CAT#: MC214629

Ust (untagged) - Mouse uronyl-2-sulfotransferase (Ust), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ust
• Synonyms: D930010O20Rik; UA2OST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214629 representing NM_177387
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGAAGCAGCAGCAGCATCCCGGCGGCGGCACGGATCCCTGGCCCCATGGGGCCCCTGTGGGGG
  GCGCCCCTCCGTGCCTGGGCAGCTGCAAGCGCCGGATCCCGCTGCTGCCTTTCCTGCGCTTCTCCCTCCG
  GGACTACGGTTTCTGCATGGCTACCCTGCTGGTCTTCTGCCTGGGCTCCCTCTTCTACCAGCTCAGCGGG
  GGACCCCCTCGCTTCCTGCTCGACCTGCGGCAGTATTTGGGAAATTCCACTTACTTGGATGACCATGGAC
  CACCTCCTAGTAAGGTCCTTCCCTTCCCCAGCCAGGTGGTGTACAACAGAGTTGGCAAGTGTGGCAGTCG
  TACCGTGGTCTTGCTTCTGAGAATCTTGTCAGAGAAGCACGGCTTCAATTTGGTCACATCAGACATTCAC
  AACAAGACCCGGCTTACTAAAAATGAACAAATGGAACTGATTAAGAATATAAGTACCGCCGAACAGCCCT
  ATTTATTCACGAGACATGTCCACTTCCTCAACTTCTCCAGGTTTGGAGGAGACCAGCCTGTCTACATCAA
  CATCATCAGAGACCCCGTCAGTAGATTTCTGTCTAACTATTTCTTCCGTCGCTTTGGAGATTGGAGAGGG
  GAACAGAATCACATGATCCGCACCCCCAGCATGAGGCAGGAGGAGCGCTACCTGGACATCAATGAGTGCA
  TTCTGGAAAACTACCCTGAGTGTTCCAACCCCAGGCTGTTTTACATCATCCCATATTTCTGTGGACAGCA
  CCCCAGATGTAGGGAGCCCGGCGAGTGGGCGCTGGAGCGGGCCAAGCTGAACGTGAACGAAAACTTCCTG
  CTCGTGGGGATCCTGGAGGAGCTGGAAGATGTGCTGCTCTTACTGGAAAGATTTTTACCTCATTACTTCA
  AGGGTGTGCTGAGCATCTACAAGGACCCAGAGCACAGGAAACTTGGCAACATGACTGTGACCGTGAGGAA
  GACTGTCCCCTCCCCCGAGGCGGTGCAGATTCTCTACCAGCGAATGAGATACGAGTACGAGTTCTACCAC
  TACGTCAGAGAACAATTCCACCTGCTCAAGCGCAAGCTTGGACTGAAGTCTCGCGTGAGCGGACCTCCCG
  TGAGACCACAGTTCTTCATCCCCACGCCCCTGGAGACGGAGGAGCCAATCGACGACGAGGAGCAAGATGA
  CGAAAAATGGCTAGAAGATATTTATAAGAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177387
• Insert Size: 1224 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177387.3, NP_796361.2
• RefSeq Size: 4284 bp
• RefSeq ORF: 1224 bp
• Locus ID: 338362
• UniProt ID: Q8BUB6
• Cytogenetics: 10 A1
• Gene Summary: Sulfotransferase that catalyzes the transfer of sulfate to the position 2 of uronyl residues. Has mainly activity toward iduronyl residues in dermatan sulfate, and weaker activity toward glucuronyl residues of chondroitin sulfate. Has no activity toward desulfated N-resulfated heparin (By similarity).[UniProtKB/Swiss-Prot Function]