A4gnt (NM_001077424) Mouse Untagged Clone

CAT#: MC214620

A4gnt (untagged) - Mouse alpha-1,4-N-acetylglucosaminyltransferase (A4gnt), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: A4gnt
• Synonyms: alph; Alpha4gnt; AV080780; Gm798
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214620 representing NM_001077424
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGGAAATTTATCTCTCCCTGTCCCTGGTCCTGGTGTTTGCCTGTGGCCTGCTGTACCAGCTCA
  CCATGAGGTCTCAGTGCTTCTTTGCCTGCCTGCCTCCTTTCTCATTTCCACAGGGACTGGACGGCCTCCT
  GAGGAGTGGACGAAGCATCATGTTTATAGAGACCTCTGAAAGGGTGGAGCCACCTCCGATGGTCTCCTGT
  GCTGTGGAGTCTGCTGCTAAAATCTATCCTGAGCAGCCCATCATCTTCTTCATGAAAGGACTCAGGGACT
  CCGTACAGCTGACTTCAAATACCAGCTACCCAGCCTTCTCCCTCCTCTCGGCCATTAATAATGTTTTCTT
  TGTGCCTTTGGACATGGAAAGACTGTTCAAAGACACACCCTTGTTTTCGTGGTACACAAAAGTCAACAGC
  AGTACAGAGAAACACTGGCTCCACGTCAGCTCCGACGCAGCCCGCCTGGCCATCATCTGGAAGTACGGAG
  GCATCTACATGGACACTGACGTCATCTCCCTTCAGCCCATCCCTGAGGAGAACTTCCTGGCAGCCCAGGG
  CTCCCGACACTCCAGTAACGGGGTATTTGGGTTCCTCCCCCACCACCCCTTCCTGTGGGCCTGCATGGAG
  AACTTTGTAGAGCACTATGACTCAACCATTTGGGGCAACCAAGGTCCCCAGTTGATGACAAGGATGTTAC
  GGGTGTGGTGTCGTCTTAAAGACTTCCACGGGTTGGGTGACCTGAAGTGTTTGAATATTTCTTTCCTTCA
  TCCCCAGAGATTTTACCCCATCCCTTATCCACAGTGGAAGCGCTACTACCAAGTGTGGGACAAAGAGCCC
  AGCTTCAATGAGTCCTACGCACTGCATTTGTGGAACTATATGAATAAAGAGGGCAAGACTGTGGTTAGAG
  GAAGCAAAACTCTGGTGGAAAATCTCTATCAAAAGCACTGTCCTAAGACTTATAGGGTTCTGATTCAAGG
  TGCAGAAGGGACAGTGTCCAAGAAGCCAGGCACAGGTTCCAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001077424
• Insert Size: 1026 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077424.2, NP_001070892.1
• RefSeq Size: 2757 bp
• RefSeq ORF: 1026 bp
• Locus ID: 333424
• UniProt ID: Q14BT6
• Cytogenetics: 9 E3.3
• Gene Summary: This gene encodes a protein from the glycosyltransferase 32 family. The encoded enzyme catalyzes the transfer of N-acetylglucosamine to alpha-1,4-linked beta-galactose residues. This enzyme is required for type III mucin synthesis and it is largely associated with the Golgi apparatus membrane. The encoded protein appears to be expressed in adenocarcinoma cells of pancreatic, biliary tract and gastric cancers.[provided by RefSeq, Jan 2010]