Ccbe1 (NM_178793) Mouse Untagged Clone

CAT#: MC214489

Ccbe1 (untagged) - Mouse collagen and calcium binding EGF domains 1 (Ccbe1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ccbe1
• Synonyms: 4933426F18Rik; 9430093N24Rik; mKIAA1983
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214489 representing NM_178793
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCGCCGCCTCTTCCGAGCCGGGGAGGAGCAGCCAAGAGGCAGCTGGGCAAGAGCCTGGGGCCGC
  TGCTGCTACTCCTGGCGCTGGGACACACGTGGACCTACCGAGAGGAACCCGAGGACCGCGACAGAGAAGT
  GTGCTCTGAAAACAAGATCACCACGACCAAATACCCATGTCTGAAGTCTTCGGGCGAGCTCACCACGTGC
  TTCAGGAAAAAATGCTGCAAAGGATATAAATTTGTTCTTGGACAGTGTATTCCAGAAGATTATGACATTT
  GTGCCCAAGCTCCCTGTGAACAGCAGTGCACGGATAACTTCGGCCGTGTGCTGTGCACGTGCTACCCGGG
  ATACCGATATGACCGCGAGAGACACCAAAAGCGGGAGAGACCGTACTGTCTGGATATCGATGAATGTGCC
  ACCAGCAATACCACTCTCTGTGCACATATCTGCATCAACACCATGGGCAGCTACCACTGTGAGTGTCGGG
  AGGGCTACATCCTTGAGGACGATGGGAGGACCTGCACAAGGGGAGACAAATACCCCAATGACACTGGGCA
  TGAAGAGAAGTCCGAGAATGAGGTGAAAGCCGGGACCTGCTGCGCCACATGCAAAGAGTTCTCCCAGATG
  AAGCAGACCGTGCTGCAGCTGAAACAGAAGATGGCGCTGCTCCCCAACAATGCCGCTGAACTGGGCAAGT
  ATGTCAATGGTGACAAGGTACTGGCCTCAAACGCCTACCTTCCAGGACCTCCTGGCCTGCCTGGGGGACA
  GGGCCCACCAGGCTCACCAGGACCCAAGGGGAGCCCTGGATTCCCCGGTATGCCAGGCCCTCCTGGACAG
  CCAGGCCCACGGGGCTCAATGGGACCTATGGGACCTTCCCCTGACCTGTCTCATATTAAGCAAGGCCGGA
  GGGGCCCCGTGGGCCCACCAGGAGCACCAGGAAGACACGGCTCCAAGGGGGAGAGAGGAGCACCCGGACC
  CCCGGGGTCTCCGGGACCCCCAGGTTCTTTCGACTTCCTGCTGCTTGTGCTGGCGGACATTCGAAATGAC
  ATCGCAGAGCTGCAGGAGAAGGTGTTTGGACACCGGACTCACTCTTCCGCCGAGGACTTCCCCCTACCTC
  AGGAATTCTCCAGCTACCCAGAGACGCTGGACTTTGGCTCTGGAGATGACTACTCCAGAAGAACTGAAGC
  AAGGGACCCCGAGGCCCCAAGAAATTTTTATCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178793
• Insert Size: 1227 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178793.4, NP_848908.1
• RefSeq Size: 5742 bp
• RefSeq ORF: 1227 bp
• Locus ID: 320924
• UniProt ID: Q3MI99
• Cytogenetics: 18 E1
• Gene Summary: Required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis.[UniProtKB/Swiss-Prot Function]