Jph4 (NM_001003829) Mouse Untagged Clone

CAT#: MC214435

Jph4 (untagged) - Mouse junctophilin 4 (Jph4), transcript variant b, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jph4
• Synonyms: 9330157P13Rik; AI425998; JP-4; JPHL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214435 representing NM_001003829
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG
  CACATGGCTACGGCGTGTGCACCGGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTT
  CGAGTCGCTGGGTGTCTTCACGGGGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGC
  GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC
  GCAGCGGCGTATGGGAGAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGG
  CTACGGCACCGAGACCTACTCGGATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGC
  TACGGGGTGCGCCAAAGTGTACCCTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGG
  ACTCCGGCCACAGCGACCCCCCGACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCC
  AGCCTCCGGCTCCCGAGGCGGCTTCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAG
  CGCACCCCAGCAGCAGGTGGATTCTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCC
  GCAGCTCCCTGGGCAGCAAGAGGGGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACC
  CCCGGGCTCCGAGGCCAGCGGGCCCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAG
  GTGTACGCCGGTGAGTGGCGTGCGGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGC
  GCTACGAAGGCGAGTGGCTGGGCAACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCG
  TGAGGAGGGCAAGTACAAGCGTAACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCC
  CTTAGACGGGGCAAAGTCAAGGAGAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTG
  CCCGCCAGCGCCAGGAAATTGCGGCTGCCAGGTGGGCATCTGGTGCACAGATCCAACTTGGGTTGAGAGG
  AGAGGTTCCTCTGGGTACTAACCACACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003829
• Insert Size: 1221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003829.2, NP_001003829.1
• RefSeq Size: 3421 bp
• RefSeq ORF: 1221 bp
• Locus ID: 319984
• Cytogenetics: 14 C3
• Gene Summary: Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH4 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.[UniProtKB/Swiss-Prot Function]