Zfyve27 (NM_001164531) Mouse Untagged Clone

CAT#: MC214422

Zfyve27 (untagged) - Mouse zinc finger, FYVE domain containing 27 (Zfyve27), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfyve27
• Synonyms: 2210011N02Rik; 9530077C24Rik; AI426636; AI593546; AI835681
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214422 representing NM_001164531
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACTTCGGATCGGGACCTGAGTGGGCCGGAGGCGAGCCCCAGCGGGATGCCTGAGGTTCTCTCTG
  AGTGTCCACCTGCCCCTACCAAGTCAGCAGCGTTTGATCTCTTCAACCTGGTCCTGTCCTACAAGAGGCT
  GGAGATCTACCTGGAACCCCTGAAGGATGCAGGGGACGGTGTTCGATACTTGCTAAGGTGGCAGATGCCT
  TTGTGTTCCTTGCTGACTTGCCTGGGCCTCAACATCTTGTTCCTCACTTTGAACGAGGGTGCGTGGTACT
  CCATGGGTGCCTTGATGATCTCGGTGCCTGCCCTACTGGGCTACCTTCAGGAGGTGTGCCGGGGACAGCT
  GCCAGAGTCGGAGCTGATGCGGAGGAAGTACCACAGCATAAGGCAGGAAGACCTGCAGAGAGTTCGCCTT
  TCCCGAGTGCACCTTTCCCGCCCTGAGGCTGTTGCTGAGGTGAAGAGCTTCTTGATCCAACTGGAAGCCT
  TCTTGGCCCGCCTGTGCTATACCTGCGAGTCAGCCTACCGTGTACTTCACTGGGAGAACCCCGTGGTGTC
  CTCACAGTTCTATGGCGCTCTTCTGGGCATGGTGTGCATGCTCTACCTGCTACCGCTCTGCTGGGTCCTC
  GCCCTGTTAAATAGCACACTCTTCCTGGGAAATGGGGACTTCTTCCGAGTGGTGTGTGAGTACAGGGCCT
  GCCTGCAGCGGAGGATGAACCCCCGGCAGGAAGAGTGTGCCTGTGAGAGCTCAGCACTGCAGGGGGCCGG
  AGGGAGGGGCCTGCTGGACAGCTCGCCTGCCCCCACACCCACCGAGGACCTCACGCCAGGCAGTGTGGAG
  GAAGCTGAGGAGGCTGAGCCAGATGAGGAGTTCAAAGATGCAATTGAGGAGGATGAAGAGGGCACCCCGT
  GCCCAGCAGAGGATGAGCTGACCCTGCAGGACAATGGATTCCTCAGCAAGAATGAGGTGCTGCGCAGCAA
  GGTGTCCCGGCTTACAGAGCGGCTTCGCAAGCGTTACCCAACCAATAACTTCGGGAATTGTGCAGGCTGT
  GCCGCTACGTTCTCCGTGCTGAAGAAGAGGCGGAGCTGCAGCAACTGTGGGAACAGCTTCTGCTCGCGGT
  GTTGCTCCTTCAAGGTGCCCAGGTCCTCTATGGGAGCCACAGCTCCTGAAGCCCAGAGAGAGACTGTGTG
  TGTGTGCGCCTCCTGCAATCAGACCCTGAGCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164531
• Insert Size: 1227 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164531.1, NP_001158003.1
• RefSeq Size: 5498 bp
• RefSeq ORF: 1227 bp
• Locus ID: 319740
• UniProt ID: Q3TXX3
• Cytogenetics: 19 C3
• Gene Summary: Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (By similarity). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (PubMed:24251978). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (By similarity). Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed:21976701).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1.